- 14-9980-80 - Invitrogen Antibodies GATA4 antibody

Submitted references Imbalanced Activation of Wnt-/β-Catenin-Signaling in Liver Endothelium Alters Normal Sinusoidal Differentiation.

Koch PS, Sandorski K, Heil J, Schmid CD, Kürschner SW, Hoffmann J, Winkler M, Staniczek T, de la Torre C, Sticht C, Schledzewski K, Taketo MM, Trogisch FA, Heineke J, Géraud C, Goerdt S, Olsavszky V
Frontiers in physiology 2021;12:722394

Naive stem cell blastocyst model captures human embryo lineage segregation.

Yanagida A, Spindlow D, Nichols J, Dattani A, Smith A, Guo G
Cell stem cell 2021 Jun 3;28(6):1016-1022.e4

Diastolic dysfunction in a pre-clinical model of diabetes is associated with changes in the cardiac non-myocyte cellular composition.

Cohen CD, De Blasio MJ, Lee MKS, Farrugia GE, Prakoso D, Krstevski C, Deo M, Donner DG, Kiriazis H, Flynn MC, Gaynor TL, Murphy AJ, Drummond GR, Pinto AR, Ritchie RH
Cardiovascular diabetology 2021 Jun 1;20(1):116

Spatiotemporal patterning of EpCAM is important for murine embryonic endo- and mesodermal differentiation.

Sarrach S, Huang Y, Niedermeyer S, Hachmeister M, Fischer L, Gille S, Pan M, Mack B, Kranz G, Libl D, Merl-Pham J, Hauck SM, Paoluzzi Tomada E, Kieslinger M, Jeremias I, Scialdone A, Gires O
Scientific reports 2018 Jan 29;8(1):1801

Genetic Intersection of Tsix and Hedgehog Signaling during the Initiation of X-Chromosome Inactivation.

Del Rosario BC, Del Rosario AM, Anselmo A, Wang PI, Sadreyev RI, Lee JT
Developmental cell 2017 Nov 6;43(3):359-371.e6

Dynamics of embryonic stem cell differentiation inferred from single-cell transcriptomics show a series of transitions through discrete cell states.

Jang S, Choubey S, Furchtgott L, Zou LN, Doyle A, Menon V, Loew EB, Krostag AR, Martinez RA, Madisen L, Levi BP, Ramanathan S
eLife 2017 Mar 15;6

CDK14 expression is down-regulated by cigarette smoke in vivo and in vitro.

Pollack D, Xiao Y, Shrivasatava V, Levy A, Andrusier M, D'Armiento J, Holz MK, Vigodner M
Toxicology letters 2015 Apr 16;234(2):120-30

Overexpression of aromatase associated with loss of heterozygosity of the STK11 gene accounts for prepubertal gynecomastia in boys with Peutz-Jeghers syndrome.

Ham S, Meachem SJ, Choong CS, Charles AK, Baynam GS, Jones TW, Samarajeewa NU, Simpson ER, Brown KA
The Journal of clinical endocrinology and metabolism 2013 Dec;98(12):E1979-87

Lentiviral transduction of rat Sertoli cells as a means to modify gene expression.

Nicholls PK, Stanton PG, Rainczuk KE, Qian H, Gregorevic P, Harrison CA
Spermatogenesis 2012 Oct 1;2(4):279-284

GATA-4 is a novel transcription factor expressed in endocardium of the developing heart.

Kelley C, Blumberg H, Zon LI, Evans T
Development (Cambridge, England) 1993 Jul;118(3):817-27

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Anti-Human/Mouse Gata-4 Purified (2 µg/mL) was used in a western blot of SDS lysates. Lane 1: NIH-3T3, Lane 2: C57Bl/6 mouse heart, Lane 3: BALB/c mouse heart.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Mouse Ovary (Lane 2) and Mouse Brain (Lane 3). The blot was probed with Anti-GATA-4 Rat Monoclonal Antibody (Product # 14-9980-82, 1:1000 dilution) and detected by chemiluminescence using F(ab')2-Rabbit anti-Rat IgG (H+L) Secondary Antibody, HRP (Product # PA1-29927, 0.25 ug/ml, 1:4000 dilution). A 50 kDa band corresponding to GATA-4 was detected across the tissues tested. Additional band around 25kDa from circulating IgGs was observed in Mouse Heart and Mouse Ovary lysates but not in Mouse Brain lysate which is selectively permeable.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 Differences in the abundance of major non-myocyte cell classes in the diabetic heart. A Flow cytometry contour plot displaying gating of major non-myocyte cell types for quantification of cell type proportion (summarised in B). For full gating strategy see Additional file 1 : Figure S2. Endothelial cells (ECs; CD31 + ), resident mesenchymal cells (RMCs; CD31 - CD45 - ) and leukocytes (Leuks; CD45 + ). B Proportions of major cell types in non-diabetic (ND; n = 7) and diabetic (DM; n = 19) mouse hearts. Individual sample values are shown with mean +- SEM. C Immunohistochemical analysis of the abundance of RMCs in ND and diabetic mouse heart left ventricles. Left and middle panels show representative confocal micrographs of mouse heart tissue stained for PCM1 and GATA4. PCM1 + GATA4 + and PCM1 - GATA4 + nuclei correspond to nuclei of cardiomyocytes (CM) and RMCs respectively. Nuclei are counterstained with DAPI. Right panel (box-plot) summarises proportion of nuclei corresponding to RMCs in ND (n = 9) vs. DM (n = 10) enumerated from micrographs. Whiskers of box-and-whisker plot indicate max and min. (D) As for C, heart left ventricle sections were stained with DACH1 to identify nuclei corresponding to endothelial cells in ND (n = 10) and DM (n = 9) left ventricles. * P < 0.05, **** P < 0.0001 (Student's unpaired t -test). Scale bar = 100 uM

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Hepatic endothelial Ctnnb1 overactivation causes loss of LSEC-associated genes and induction of CEC and brain EC genes. (A) IF staining of DAPI, LYVE1, and Emcn in the liver of 2- to 3-month-old female Ctnnb1 WT and Ctnnb1 OE - EC mice ( n = 4). Scale bar 100 mum. (B) Left panel: IF quantification of LYVE1 + area. Results are represented as mean +- SEM. ** p < 0.01. Right panel: qRT-PCR for LYVE1 of cDNA from Ctnnb1 OE - EC -LSECs compared to Ctnnb1 WT control LSECs ( n = 4). beta-Actin was used as housekeeping gene. * p < 0.05. (C) Bmp2, Hgf , Wnt2, Wnt9b mRNA RNAScope in situ hybridization assay of 2- to 3-month-old female Ctnnb1 WT and Ctnnb1 OE - EC mice liver sections ( n >= 3). Scale bar 100 mum. (D) Quantification of Bmp2, Hgf , Wnt2, Wnt9b mRNA RNAScope in situ hybridization assay. Results are represented as mean +- SEM. ns, not significant. * p < 0.05. (E) Left panel: IF staining of DAPI and GATA4 in the liver of 2- to 3-month-old female Ctnnb1 WT and Ctnnb1 OE - EC mice ( n = 4). Scale bar 100 mum. Right panel: qRT-PCR for Gata4 with cDNA from Ctnnb1 OE - EC -LSECs compared to Ctnnb1 WT control LSECs ( n = 4). beta-Actin was used as housekeeping gene. n.s., not significant. (F) qRT-PCR for Myc and Pdgfb with cDNA from Ctnnb1 OE - EC -LSECs compared with Ctnnb1 WT control LSECs ( n = 3). beta-Actin was used as housekeeping gene. * , p < 0.05; n.s., not significant. (G) qRT-PCR for CD34 with cDNA from Ctnnb1 OE - EC -LSECs compared with Ctnnb1 WT control LSEC

14-9980-80

Antibody data