MAB7352

antibody from Novus Biologicals

Targeting: IL10 CSIF, IL-10, IL10A, TGIF

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB7352

Provider

Novus Biologicals

Product name

Mouse Monoclonal IL-10 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects canine IL-10 in Western blots.

Reactivity

Canine

Host

Mouse

Isotype

IgG

Vial size

500 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Recovery of antigen-specific T cell responses from dogs infected with Leishmania (L.) infantum by use of vaccine associated TLR-agonist adjuvant.

Schaut RG, Grinnage-Pulley TL, Esch KJ, Toepp AJ, Duthie MS, Howard RF, Reed SG, Petersen CA
Vaccine 2016 Oct 17;34(44):5225-5234

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Expression of Toll-like receptors 2 and 9 in cells of dog jejunum and colon naturally infected with Leishmania infantum.

Figueiredo MM, Amorim IF, Pinto AJ, Barbosa VS, Pinheiro Lde J, Deoti B, Faria AM, Tafuri WL
BMC immunology 2013 May 14;14:22

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Cell Proliferation Induced by IL-10 and Neutralization by Canine IL-10 Antibody. Recombinant Canine IL-10 (Catalog # 735-CL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-10 (2 ng/mL) is neutralized (green line) by increasing concentrations of Canine IL-10 Monoclonal Antibody (Catalog # MAB7352). The ND₅₀ is typically 0.1-0.25 µg/mL.