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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Blocking/Neutralizing [1]
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- Product number
- MAB2171-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human IL-10 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant mouse IL-10.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P22301- Isotype
- IgG
- Antibody clone number
- 25209
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Oral fibroblasts modulate the macrophage response to bacterial challenge.
Interleukin-10-producing LAG3+ regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis.
S100A8/A9 mRNA induction in an ex vivo model of endotoxin tolerance: roles of IL-10 and IFNγ.
HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms.
IFN-γ-rich environment programs dendritic cells toward silencing of cytotoxic immune responses.
Monocytes induce STAT3 activation in human mesenchymal stem cells to promote osteoblast formation.
Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function.
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Effect of IL-21 on NK cells derived from different umbilical cord blood populations.
Tzach-Nahman R, Nashef R, Fleissig O, Palmon A, Shapira L, Wilensky A, Nussbaum G
Scientific reports 2017 Sep 14;7(1):11516
Scientific reports 2017 Sep 14;7(1):11516
Interleukin-10-producing LAG3+ regulatory T cells are associated with disease activity and abatacept treatment in rheumatoid arthritis.
Nakachi S, Sumitomo S, Tsuchida Y, Tsuchiya H, Kono M, Kato R, Sakurai K, Hanata N, Nagafuchi Y, Tateishi S, Kanda H, Okamura T, Yamamoto K, Fujio K
Arthritis research & therapy 2017 May 16;19(1):97
Arthritis research & therapy 2017 May 16;19(1):97
S100A8/A9 mRNA induction in an ex vivo model of endotoxin tolerance: roles of IL-10 and IFNγ.
Fontaine M, Planel S, Peronnet E, Turrel-Davin F, Piriou V, Pachot A, Monneret G, Lepape A, Venet F
PloS one 2014;9(6):e100909
PloS one 2014;9(6):e100909
HIV-1 Tat protein induces PD-L1 (B7-H1) expression on dendritic cells through tumor necrosis factor alpha- and toll-like receptor 4-mediated mechanisms.
Planès R, BenMohamed L, Leghmari K, Delobel P, Izopet J, Bahraoui E
Journal of virology 2014 Jun;88(12):6672-89
Journal of virology 2014 Jun;88(12):6672-89
IFN-γ-rich environment programs dendritic cells toward silencing of cytotoxic immune responses.
Svajger U, Obermajer N, Jeras M
Journal of leukocyte biology 2014 Jan;95(1):33-46
Journal of leukocyte biology 2014 Jan;95(1):33-46
Monocytes induce STAT3 activation in human mesenchymal stem cells to promote osteoblast formation.
Nicolaidou V, Wong MM, Redpath AN, Ersek A, Baban DF, Williams LM, Cope AP, Horwood NJ
PloS one 2012;7(7):e39871
PloS one 2012;7(7):e39871
Analysis of adhesion molecules, target cells, and role of IL-2 in human FOXP3+ regulatory T cell suppressor function.
Tran DQ, Glass DD, Uzel G, Darnell DA, Spalding C, Holland SM, Shevach EM
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 1;182(5):2929-38
Journal of immunology (Baltimore, Md. : 1950) 2009 Mar 1;182(5):2929-38
Distinct subsets of human invariant NKT cells differentially regulate T helper responses via dendritic cells.
Liu TY, Uemura Y, Suzuki M, Narita Y, Hirata S, Ohyama H, Ishihara O, Matsushita S
European journal of immunology 2008 Apr;38(4):1012-23
European journal of immunology 2008 Apr;38(4):1012-23
Effect of IL-21 on NK cells derived from different umbilical cord blood populations.
Perez SA, Mahaira LG, Sotiropoulou PA, Gritzapis AD, Iliopoulou EG, Niarchos DK, Cacoullos NT, Kavalakis YG, Antsaklis AI, Sotiriadou NN, Baxevanis CN, Papamichail M
International immunology 2006 Jan;18(1):49-58
International immunology 2006 Jan;18(1):49-58
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human IL-10 by Western Blot. Western blot shows conditioned media of HEK293 human embryonic kidney cell line either mock transfected or transfected with human IL-10. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2171) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for IL-10 at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Cell Proliferation Induced by IL-10 and Neutralization by Human IL-10 Antibody. Recombinant Human IL-10 (Catalog # 217-IL) stimulates proliferation in the MC/9-2 mouse mast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-10 (5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-10 Monoclonal Antibody (Catalog # MAB2171). The ND50 is typically 0.005-0.03 µg/mL.
