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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- 506811 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#506811, RRID:AB_2632782
- Product name
- PE/Dazzle™ 594 anti-human IL-10
- Antibody type
- Monoclonal
- Description
- IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES3-19F1 antibody reacts with human and viral interleukin-10 (IL-10). The JES3-19F1 antibody can neutralize the bioactivity of natural or recombinant IL-10.
- Reactivity
- Human
- Host
- Rat
- Conjugate
- Red dye
- Antibody clone number
- JES3-19F1
- Vial size
- 25 tests
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- LPS-stimulated (in the presence of monensin, 18 hours) human peripheral blood mononuclear cells were surface stained with CD14 APC, then fixed with Fixation Buffer permeabilized with Permeabilization Wash Buffer and intracellularly stained with anti-human IL-10 (clone JES3-19F1) PE/Dazzle™ 594 (top) or rat IgG2a, κ PE/Dazzle™ 594 isotype control (bottom). Data shown was gated on monocyte population.
