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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 50-7108-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-IL-10 Monoclonal Antibody (JES3-9D7), eFluor 660, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The JES3-9D7 monoclonal antibody reacts with human interleukin-10 (IL-10). Applications Reported: This JES3-9D7 antibody has been reported for use in flow cytometric analysis, and intracellular staining followed by flow cytometric analysis. Applications Tested: This JES3-9D7 antibody has been pre-titrated and tested by intracellular staining anf flow cytometryci analysis of Th2 polarized and stimulated human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human
- Host
- Rat
- Isotype
- IgG
- Antibody clone number
- JES3-9D7
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Multiple Sclerosis-Associated Changes in the Composition and Immune Functions of Spore-Forming Bacteria.
Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.
Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues.
The regulation of cancer cell migration by lung cancer cell-derived exosomes through TGF-β and IL-10.
Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.
Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.
Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.
Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.
Role of the phosphatidylinositol 3 kinase-Akt pathway in the regulation of IL-10 and IL-12 by Porphyromonas gingivalis lipopolysaccharide.
Cekanaviciute E, Pröbstel AK, Thomann A, Runia TF, Casaccia P, Katz Sand I, Crabtree E, Singh S, Morrissey J, Barba P, Gomez R, Knight R, Mazmanian S, Graves J, Cree BAC, Zamvil SS, Baranzini SE
mSystems 2018 Nov-Dec;3(6)
mSystems 2018 Nov-Dec;3(6)
Heterologous Immunity between Adenoviruses and Hepatitis C Virus: A New Paradigm in HCV Immunity and Vaccines.
Singh S, Vedi S, Samrat SK, Li W, Kumar R, Agrawal B
PloS one 2016;11(1):e0146404
PloS one 2016;11(1):e0146404
Functional expression of aryl hydrocarbon receptor on mast cells populating human endometriotic tissues.
Mariuzzi L, Domenis R, Orsaria M, Marzinotto S, Londero AP, Bulfoni M, Candotti V, Zanello A, Ballico M, Mimmi MC, Calcagno A, Marchesoni D, Di Loreto C, Beltrami AP, Cesselli D, Gri G
Laboratory investigation; a journal of technical methods and pathology 2016 Sep;96(9):959-971
Laboratory investigation; a journal of technical methods and pathology 2016 Sep;96(9):959-971
The regulation of cancer cell migration by lung cancer cell-derived exosomes through TGF-β and IL-10.
Wang Y, Yi J, Chen X, Zhang Y, Xu M, Yang Z
Oncology letters 2016 Feb;11(2):1527-1530
Oncology letters 2016 Feb;11(2):1527-1530
Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.
Miles B, Miller SM, Folkvord JM, Kimball A, Chamanian M, Meditz AL, Arends T, McCarter MD, Levy DN, Rakasz EG, Skinner PJ, Connick E
Nature communications 2015 Oct 20;6:8608
Nature communications 2015 Oct 20;6:8608
Granzyme B production distinguishes recently activated CD8(+) memory cells from resting memory cells.
Nowacki TM, Kuerten S, Zhang W, Shive CL, Kreher CR, Boehm BO, Lehmann PV, Tary-Lehmann M
Cellular immunology 2007 May;247(1):36-48
Cellular immunology 2007 May;247(1):36-48
Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.
Sendide K, Deghmane AE, Pechkovsky D, Av-Gay Y, Talal A, Hmama Z
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):5324-32
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):5324-32
Mycobacterium bovis BCG attenuates surface expression of mature class II molecules through IL-10-dependent inhibition of cathepsin S.
Sendide K, Deghmane AE, Pechkovsky D, Av-Gay Y, Talal A, Hmama Z
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):5324-32
Journal of immunology (Baltimore, Md. : 1950) 2005 Oct 15;175(8):5324-32
Role of the phosphatidylinositol 3 kinase-Akt pathway in the regulation of IL-10 and IL-12 by Porphyromonas gingivalis lipopolysaccharide.
Martin M, Schifferle RE, Cuesta N, Vogel SN, Katz J, Michalek SM
Journal of immunology (Baltimore, Md. : 1950) 2003 Jul 15;171(2):717-25
Journal of immunology (Baltimore, Md. : 1950) 2003 Jul 15;171(2):717-25
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CD4+ human peripheral blood cells were polarized under Th2 conditions then were unstimulated (left) or stimulated for 5 hours with Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03). Cells were intracellularly stained with Anti-Human CD4 FITC (Product # 11-0049-42) and Anti-Human IL-10 eFluor® 660 using the Fixation & Permeabilization Buffers (Product # 88-8824-00). Total viable cells were used for analysis.
