501419

antibody from BioLegend

Targeting: IL10 CSIF, IL-10, IL10A, TGIF

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

501419

Provider

BioLegend

Proper citation

BioLegend Cat#501419, RRID:AB_2280292

Product name

PE/Cy7 anti-human IL-10

Antibody type

Monoclonal

Antigen

COS - expressed, recombinant human IL-10

Description

IL-10 was originally described as Cytokine Synthesis Inhibitory Factor (CSIF) by virtue of its ability to inhibit cytokine production by Th1 clones. IL-10 shares over 80% sequence homology with the Epstein-Barr virus protein BCRFI. The biological activities of IL-10 include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed type hypersensitivity response, and stimulation of the Th2 cell response, which results in elevated antibody production. The JES3-9D7 antibody reacts with human and viral interleukin-10 (IL-10).

Reactivity

Human, Simian

Host

Rat

Conjugate

Infrared dye

Isotype

IgG

Vial size

25 tests

Storage

The antibody solution should be stored undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

References

Submitted references

Glucocorticosteroids modify Langerhans cells to produce TGF-β and expand regulatory T cells.

Stary G, Klein I, Bauer W, Koszik F, Reininger B, Kohlhofer S, Gruber K, Skvara H, Jung T, Stingl G
Journal of immunology (Baltimore, Md. : 1950) 2011 Jan 1;186(1):103-12

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

LPS-stimulated (overnight) human peripheral blood mononuclear cells surface stained with CD14 APC and intracellularly stained with JES3-9D7 PE/Cy7 (top data) or rat IgG1 isotype control PE/Cy7 (bottom data) (gated on monocyte population)

Conjugate

Near infrared dye