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LearnNBP2-27149
antibody from Novus Biologicals
Targeting: TLR4
ARMD10, CD284, hToll, TLR-4
Western blot
Immunocytochemistry Immunohistochemistry Flow cytometry Blocking/Neutralizing Chromatin ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [24]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
- Flow cytometry [8]
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- Product number
- NBP2-27149 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal TLR4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. The amino acid sequence used has 100% identity in human, chimp, baboon, chinese hamster, and rat
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references TLR4 has a TP53-dependent dual role in regulating breast cancer cell growth.
Prostaglandin E2 reduces Toll-like receptor 4 expression in alveolar macrophages by inhibition of translation.
Characterization of the Toll-like receptor expression profile in human multiple myeloma cells.
Changes of the enteric nervous system in amyloid-β protein precursor transgenic mice correlate with disease progression.
Cirrhosis is associated with development of tolerance to cardiac chronotropic effect of endotoxin in rats.
Ablation of the cellular prion protein, PrPC, specifically on follicular dendritic cells has no effect on their maturation or function.
Toll-like receptor-4 agonist inhibits motility and invasion of hepatoblastoma HepG2 cells in vitro.
Differential expression of TLR-2 and TLR-4 in the epithelial cells in oral lichen planus.
CD74 and macrophage migration inhibitory factor as therapeutic targets in gastric cancer.
Lactobacillus reuteri strains reduce incidence and severity of experimental necrotizing enterocolitis via modulation of TLR4 and NF-κB signaling in the intestine.
Altered sciatic nerve fiber morphology and endoneural microvessels in mouse models relevant for obesity, peripheral diabetic polyneuropathy, and the metabolic syndrome.
Glucose activation of islets of Langerhans up-regulates Toll-like receptor 5: possible mechanism of protection.
Tissue-resident mesenchymal stem cells attract peripheral blood neutrophils and enhance their inflammatory activity in response to microbial challenge.
The K5 capsule of Escherichia coli strain Nissle 1917 is important in stimulating expression of Toll-like receptor 5, CD14, MyD88, and TRIF together with the induction of interleukin-8 expression via the mitogen-activated protein kinase pathway in epithelial cells.
Extracts of the rat tapeworm, Hymenolepis diminuta, suppress macrophage activation in vitro and alleviate chemically induced colitis in mice.
Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific.
Oxidized low-density lipoprotein (oxLDL)-induced cell death in dorsal root ganglion cell cultures depends not on the lectin-like oxLDL receptor-1 but on the toll-like receptor-4.
Binding of different forms of lipopolysaccharide and gene expression in bovine blood neutrophils.
Antiflagellin antibodies recognize the autoantigens Toll-Like Receptor 5 and Pals 1-associated tight junction protein and induce monocytes activation and increased intestinal permeability in Crohn's disease.
Phosphodiesterase 4 inhibition but not beta-adrenergic stimulation suppresses tumor necrosis factor-alpha release in peripheral blood mononuclear cells in septic shock.
STAT3- and STAT5-dependent pathways competitively regulate the pan-differentiation of CD34pos cells into tumor-competent dendritic cells.
CD19 regulates skin and lung fibrosis via Toll-like receptor signaling in a model of bleomycin-induced scleroderma.
Toll-like receptor 4 ligation enforces tolerogenic properties of oral mucosal Langerhans cells.
In celiac disease, a subset of autoantibodies against transglutaminase binds toll-like receptor 4 and induces activation of monocytes.
Haricharan S, Brown P
Proceedings of the National Academy of Sciences of the United States of America 2015 Jun 23;112(25):E3216-25
Proceedings of the National Academy of Sciences of the United States of America 2015 Jun 23;112(25):E3216-25
Prostaglandin E2 reduces Toll-like receptor 4 expression in alveolar macrophages by inhibition of translation.
Degraaf AJ, Zasłona Z, Bourdonnay E, Peters-Golden M
American journal of respiratory cell and molecular biology 2014 Aug;51(2):242-50
American journal of respiratory cell and molecular biology 2014 Aug;51(2):242-50
Characterization of the Toll-like receptor expression profile in human multiple myeloma cells.
Abdi J, Mutis T, Garssen J, Redegeld F
PloS one 2013;8(4):e60671
PloS one 2013;8(4):e60671
Changes of the enteric nervous system in amyloid-β protein precursor transgenic mice correlate with disease progression.
Semar S, Klotz M, Letiembre M, Van Ginneken C, Braun A, Jost V, Bischof M, Lammers WJ, Liu Y, Fassbender K, Wyss-Coray T, Kirchhoff F, Schäfer KH
Journal of Alzheimer's disease : JAD 2013;36(1):7-20
Journal of Alzheimer's disease : JAD 2013;36(1):7-20
Cirrhosis is associated with development of tolerance to cardiac chronotropic effect of endotoxin in rats.
Jazaeri F, Tavangar SM, Ghazi-Khansari M, Khorramizadeh MR, Mani AR, Dehpour AR
Liver international : official journal of the International Association for the Study of the Liver 2013 Mar;33(3):368-74
Liver international : official journal of the International Association for the Study of the Liver 2013 Mar;33(3):368-74
Ablation of the cellular prion protein, PrPC, specifically on follicular dendritic cells has no effect on their maturation or function.
McCulloch L, Brown KL, Mabbott NA
Immunology 2013 Mar;138(3):246-57
Immunology 2013 Mar;138(3):246-57
Toll-like receptor-4 agonist inhibits motility and invasion of hepatoblastoma HepG2 cells in vitro.
Hsiao CC, Kao YH, Huang SC, Chuang JH
Pediatric blood & cancer 2013 Feb;60(2):248-53
Pediatric blood & cancer 2013 Feb;60(2):248-53
Differential expression of TLR-2 and TLR-4 in the epithelial cells in oral lichen planus.
Janardhanam SB, Prakasam S, Swaminathan VT, Kodumudi KN, Zunt SL, Srinivasan M
Archives of oral biology 2012 May;57(5):495-502
Archives of oral biology 2012 May;57(5):495-502
CD74 and macrophage migration inhibitory factor as therapeutic targets in gastric cancer.
Zheng YX, Yang M, Rong TT, Yuan XL, Ma YH, Wang ZH, Shen LS, Cui L
World journal of gastroenterology 2012 May 14;18(18):2253-61
World journal of gastroenterology 2012 May 14;18(18):2253-61
Lactobacillus reuteri strains reduce incidence and severity of experimental necrotizing enterocolitis via modulation of TLR4 and NF-κB signaling in the intestine.
Liu Y, Fatheree NY, Mangalat N, Rhoads JM
American journal of physiology. Gastrointestinal and liver physiology 2012 Mar 15;302(6):G608-17
American journal of physiology. Gastrointestinal and liver physiology 2012 Mar 15;302(6):G608-17
Altered sciatic nerve fiber morphology and endoneural microvessels in mouse models relevant for obesity, peripheral diabetic polyneuropathy, and the metabolic syndrome.
Nowicki M, Kosacka J, Serke H, Blüher M, Spanel-Borowski K
Journal of neuroscience research 2012 Jan;90(1):122-31
Journal of neuroscience research 2012 Jan;90(1):122-31
Glucose activation of islets of Langerhans up-regulates Toll-like receptor 5: possible mechanism of protection.
Weile C, Josefsen K, Buschard K
Clinical and experimental immunology 2011 Nov;166(2):251-7
Clinical and experimental immunology 2011 Nov;166(2):251-7
Tissue-resident mesenchymal stem cells attract peripheral blood neutrophils and enhance their inflammatory activity in response to microbial challenge.
Brandau S, Jakob M, Hemeda H, Bruderek K, Janeschik S, Bootz F, Lang S
Journal of leukocyte biology 2010 Nov;88(5):1005-15
Journal of leukocyte biology 2010 Nov;88(5):1005-15
The K5 capsule of Escherichia coli strain Nissle 1917 is important in stimulating expression of Toll-like receptor 5, CD14, MyD88, and TRIF together with the induction of interleukin-8 expression via the mitogen-activated protein kinase pathway in epithelial cells.
Hafez M, Hayes K, Goldrick M, Grencis RK, Roberts IS
Infection and immunity 2010 May;78(5):2153-62
Infection and immunity 2010 May;78(5):2153-62
Extracts of the rat tapeworm, Hymenolepis diminuta, suppress macrophage activation in vitro and alleviate chemically induced colitis in mice.
Johnston MJ, Wang A, Catarino ME, Ball L, Phan VC, MacDonald JA, McKay DM
Infection and immunity 2010 Mar;78(3):1364-75
Infection and immunity 2010 Mar;78(3):1364-75
Toll-like receptor-induced innate immune responses in non-parenchymal liver cells are cell type-specific.
Wu J, Meng Z, Jiang M, Zhang E, Trippler M, Broering R, Bucchi A, Krux F, Dittmer U, Yang D, Roggendorf M, Gerken G, Lu M, Schlaak JF
Immunology 2010 Mar;129(3):363-74
Immunology 2010 Mar;129(3):363-74
Oxidized low-density lipoprotein (oxLDL)-induced cell death in dorsal root ganglion cell cultures depends not on the lectin-like oxLDL receptor-1 but on the toll-like receptor-4.
Nowicki M, Müller K, Serke H, Kosacka J, Vilser C, Ricken A, Spanel-Borowski K
Journal of neuroscience research 2010 Feb 1;88(2):403-12
Journal of neuroscience research 2010 Feb 1;88(2):403-12
Binding of different forms of lipopolysaccharide and gene expression in bovine blood neutrophils.
Worku M, Morris A
Journal of dairy science 2009 Jul;92(7):3185-93
Journal of dairy science 2009 Jul;92(7):3185-93
Antiflagellin antibodies recognize the autoantigens Toll-Like Receptor 5 and Pals 1-associated tight junction protein and induce monocytes activation and increased intestinal permeability in Crohn's disease.
Lunardi C, Bason C, Dolcino M, Navone R, Simone R, Saverino D, Frulloni L, Tinazzi E, Peterlana D, Corrocher R, Puccetti A
Journal of internal medicine 2009 Feb;265(2):250-65
Journal of internal medicine 2009 Feb;265(2):250-65
Phosphodiesterase 4 inhibition but not beta-adrenergic stimulation suppresses tumor necrosis factor-alpha release in peripheral blood mononuclear cells in septic shock.
Link A, Selejan S, Maack C, Lenz M, Böhm M
Critical care (London, England) 2008;12(6):R159
Critical care (London, England) 2008;12(6):R159
STAT3- and STAT5-dependent pathways competitively regulate the pan-differentiation of CD34pos cells into tumor-competent dendritic cells.
Cohen PA, Koski GK, Czerniecki BJ, Bunting KD, Fu XY, Wang Z, Zhang WJ, Carter CS, Awad M, Distel CA, Nagem H, Paustian CC, Johnson TD, Tisdale JF, Shu S
Blood 2008 Sep 1;112(5):1832-43
Blood 2008 Sep 1;112(5):1832-43
CD19 regulates skin and lung fibrosis via Toll-like receptor signaling in a model of bleomycin-induced scleroderma.
Yoshizaki A, Iwata Y, Komura K, Ogawa F, Hara T, Muroi E, Takenaka M, Shimizu K, Hasegawa M, Fujimoto M, Tedder TF, Sato S
The American journal of pathology 2008 Jun;172(6):1650-63
The American journal of pathology 2008 Jun;172(6):1650-63
Toll-like receptor 4 ligation enforces tolerogenic properties of oral mucosal Langerhans cells.
Allam JP, Peng WM, Appel T, Wenghoefer M, Niederhagen B, Bieber T, Bergé S, Novak N
The Journal of allergy and clinical immunology 2008 Feb;121(2):368-374.e1
The Journal of allergy and clinical immunology 2008 Feb;121(2):368-374.e1
In celiac disease, a subset of autoantibodies against transglutaminase binds toll-like receptor 4 and induces activation of monocytes.
Zanoni G, Navone R, Lunardi C, Tridente G, Bason C, Sivori S, Beri R, Dolcino M, Valletta E, Corrocher R, Puccetti A
PLoS medicine 2006 Sep;3(9):e358
PLoS medicine 2006 Sep;3(9):e358
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLR4 Antibody (76B357.1) - Azide Free [NBP2-27149] - Analysis of TLR4 using TLR4 antibody at 2 ug/ml on (A) human intestine and 6 ug/ml on (B) mouse intestine and C) rat intestine lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) - Azide Free [NBP2-27149] - Formalin-fixed, paraffin-embedded human testis tissue stained with TLR4 antibody (5 ug/ml).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TLR4 Antibody (76B357.1) - Azide Free [NBP2-27149] - Human skin stained with TLR4 antibody (5 ug/ml), peroxidase-conjugate and DAB chromogen.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (76B357.1) - Azide Free [NBP2-27149] - Analysis using PE conjugate of NBP2-27149. An intracellular stain was performed on Jurkat cells with TLR4 antibody (76B357.1) NBP2-27149PE (blue) and an isotype control MAB004 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (76B357.1) - Azide Free [NBP2-27149] - Analysis using the Alexa Fluor (R) 647 conjugate of NBP2-27149. TLR4 expression on monocytes from human peripheral blood: PBMC were stained, in a 2 color flow test, with CD14 PE this antibody and 1 ug of either isotype control (Left, ) or TLR4-Alexa Fluor 647 (right). PPI negative, CD14+ cells were gated for analysis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (76B357.1) - Azide Free [NBP2-27149] - Analysis using the FITC conjugate of NBP2-27149. TLR4 expression on monocytes from human peripheral blood: PBMC were stained, in a 2 color flow test, with CD14 PE and 1 ug of either isotype control (Left, ) or TLR4-FITC (Right, this antibody. PPI negative, CD14+ cells were gated for analysis.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (76B357.1) - Azide Free [NBP2-27149] - Analysis using the FITC conjugate of NBP2-27149. Staining of cell surface TLR4 in human monocyte-derived dendritic cells using this antibody at 1:200 (2.5 ug). Black and green open histograms represent unstained cells and negative control (anti-human CD14
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (76B357.1) - Azide Free [NBP2-27149] - Analysis of cell surface TLR4 in mouse splenocytes using 0.5 ug/10^6 cells of TLR4 antibody. Shaded histogram represents cells without TLR4 antibody; green represents isotype control (BD Pharmingen) red represents TLR4 antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (76B357.1) - Azide Free [NBP2-27149] - An intracellular stain was performed on Jurkat cells with TLR4 antibody (76B357.1) NBP2-27149AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (76B357.1) - Azide Free [NBP2-27149] - An intracellular stain was performed on Jurkat cells with TLR4 antibody (76B357.1) NBP2-27149APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Allophycocyanin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: TLR4 Antibody (76B357.1) - Azide Free [NBP2-27149] - An intracellular stain was performed on Jurkat cells with TLR4 antibody (76B357.1) NBP2-27149AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
