Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- NB100-56579 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56579, RRID:AB_839007
- Product name
- Rabbit Polyclonal TLR4 Antibody
- Antibody type
- Polyclonal
- Description
- Protein G purified. Observed size: approx. 90 kDa (native) or 80 kDa (partial recombinant mouse TLR4, extracellular portion plus His-tag)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Biglycan-triggered TLR-2- and TLR-4-signaling exacerbates the pathophysiology of ischemic acute kidney injury.
Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages.
Regulation of Toll-like receptor 2 interaction with Ecgp96 controls Escherichia coli K1 invasion of brain endothelial cells.
Signaling by the matrix proteoglycan decorin controls inflammation and cancer through PDCD4 and MicroRNA-21.
TLR4-mediated activation of mouse macrophages by Korean mistletoe lectin-C (KML-C).
PE_PGRS antigens of Mycobacterium tuberculosis induce maturation and activation of human dendritic cells.
Tetraspanin CD9 negatively regulates lipopolysaccharide-induced macrophage activation and lung inflammation.
Role of APOBEC3G/F-mediated hypermutation in the control of human immunodeficiency virus type 1 in elite suppressors.
Intracellularly expressed TLR2s and TLR4s contribution to an immunosilent environment at the ocular mucosal epithelium.
Intracellularly expressed TLR2s and TLR4s contribution to an immunosilent environment at the ocular mucosal epithelium.
Moreth K, Frey H, Hubo M, Zeng-Brouwers J, Nastase MV, Hsieh LT, Haceni R, Pfeilschifter J, Iozzo RV, Schaefer L
Matrix biology : journal of the International Society for Matrix Biology 2014 Apr;35:143-51
Matrix biology : journal of the International Society for Matrix Biology 2014 Apr;35:143-51
Statins decrease lung inflammation in mice by upregulating tetraspanin CD9 in macrophages.
Jin Y, Tachibana I, Takeda Y, He P, Kang S, Suzuki M, Kuhara H, Tetsumoto S, Tsujino K, Minami T, Iwasaki T, Nakanishi K, Kohmo S, Hirata H, Takahashi R, Inoue K, Nagatomo I, Kida H, Kijima T, Ito M, Saya H, Kumanogoh A
PloS one 2013;8(9):e73706
PloS one 2013;8(9):e73706
Regulation of Toll-like receptor 2 interaction with Ecgp96 controls Escherichia coli K1 invasion of brain endothelial cells.
Krishnan S, Chen S, Turcatel G, Arditi M, Prasadarao NV
Cellular microbiology 2013 Jan;15(1):63-81
Cellular microbiology 2013 Jan;15(1):63-81
Signaling by the matrix proteoglycan decorin controls inflammation and cancer through PDCD4 and MicroRNA-21.
Merline R, Moreth K, Beckmann J, Nastase MV, Zeng-Brouwers J, Tralhão JG, Lemarchand P, Pfeilschifter J, Schaefer RM, Iozzo RV, Schaefer L
Science signaling 2011 Nov 15;4(199):ra75
Science signaling 2011 Nov 15;4(199):ra75
TLR4-mediated activation of mouse macrophages by Korean mistletoe lectin-C (KML-C).
Park HJ, Hong JH, Kwon HJ, Kim Y, Lee KH, Kim JB, Song SK
Biochemical and biophysical research communications 2010 Jun 4;396(3):721-5
Biochemical and biophysical research communications 2010 Jun 4;396(3):721-5
PE_PGRS antigens of Mycobacterium tuberculosis induce maturation and activation of human dendritic cells.
Bansal K, Elluru SR, Narayana Y, Chaturvedi R, Patil SA, Kaveri SV, Bayry J, Balaji KN
Journal of immunology (Baltimore, Md. : 1950) 2010 Apr 1;184(7):3495-504
Journal of immunology (Baltimore, Md. : 1950) 2010 Apr 1;184(7):3495-504
Tetraspanin CD9 negatively regulates lipopolysaccharide-induced macrophage activation and lung inflammation.
Suzuki M, Tachibana I, Takeda Y, He P, Minami S, Iwasaki T, Kida H, Goya S, Kijima T, Yoshida M, Kumagai T, Osaki T, Kawase I
Journal of immunology (Baltimore, Md. : 1950) 2009 May 15;182(10):6485-93
Journal of immunology (Baltimore, Md. : 1950) 2009 May 15;182(10):6485-93
Role of APOBEC3G/F-mediated hypermutation in the control of human immunodeficiency virus type 1 in elite suppressors.
Gandhi SK, Siliciano JD, Bailey JR, Siliciano RF, Blankson JN
Journal of virology 2008 Mar;82(6):3125-30
Journal of virology 2008 Mar;82(6):3125-30
Intracellularly expressed TLR2s and TLR4s contribution to an immunosilent environment at the ocular mucosal epithelium.
Ueta M, Nochi T, Jang MH, Park EJ, Igarashi O, Hino A, Kawasaki S, Shikina T, Hiroi T, Kinoshita S, Kiyono H
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 1;173(5):3337-47
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 1;173(5):3337-47
Intracellularly expressed TLR2s and TLR4s contribution to an immunosilent environment at the ocular mucosal epithelium.
Ueta M, Nochi T, Jang MH, Park EJ, Igarashi O, Hino A, Kawasaki S, Shikina T, Hiroi T, Kinoshita S, Kiyono H
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 1;173(5):3337-47
Journal of immunology (Baltimore, Md. : 1950) 2004 Sep 1;173(5):3337-47
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TLR4 Antibody [NB100-56579] - Analysis of TLR4 using this antibody on (A) 1 ug/lane recombinant mouse TLR4 protein (tested at 2 ug/mL), (B) 20 ug/lane human intestine, and (C) 20 ug/lane mouse intestine (tested at 5 ug/mL on intestine lysates).