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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- GTX17944 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX17944, RRID:AB_422987
- Product name
- RPS6 (phospho Ser236) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Lysates prepared from HEK293 cells left untreated (1) or treated with EGF (2-6) were resolved by SDS-PAGE on a 14% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer for one hour at room temperature, and incubated with ribosomal protein S6 [pS236] antibody for two hours at room temperature in a 3% BSA-TBST buffer, following prior incubation with: no peptide (1, 2), the phosphopeptide corresponding to ribosomal protein S6 [pSpS244/247] (3), the non-phosphopeptide corresponding to the immunogen (4), a generic phosphoserine-containing peptide (5), or, the phosphopeptide immunogen (6). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to ribosomal protein S6 [pS236] blocks the signal, verifying the specificity of the antibody.
