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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Flow cytometry [1]
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- Product number
- 12-9007-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-S6 (Ser235, Ser236) Monoclonal Antibody (cupk43k), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This cupk43k monoclonal antibody recognizes human and mouse ribosomal protein S6 (also known as 40S ribosomal protein S6, phosphoprotein NP33, RPS6, RS6, S6) when phosphorylated on serine 235 (S235, human) and serine 236 (S236, mouse). Ribosomal protein S6 is a component of the 40S subunit of the ribosome and is phosphorylated at multiple sites following stimulation of cells by growth factors, tumor promoting agents, or mitogens. Phosphorylation of ribosomal protein S6 by p70S6K and PKDCD results in upregulation of the translation of RNA coding for proteins involved in cell cycle entry. Ribosomal protein S6 is dephosphorylated upon growth arrest.
- Conjugate
- Yellow dye
- Antibody clone number
- cupk43k
- Concentration
- 5 µL/Test
Submitted references Cbl Ubiquitin Ligases Control B Cell Exit from the Germinal-Center Reaction.
Autophagy orchestrates the regulatory program of tumor-associated myeloid-derived suppressor cells.
PTEN drives Th17 cell differentiation by preventing IL-2 production.
Dichotomous Expression of TNF Superfamily Ligands on Antigen-Presenting Cells Controls Post-priming Anti-viral CD4(+) T Cell Immunity.
Intestinal Epithelial and Intraepithelial T Cell Crosstalk Mediates a Dynamic Response to Infection.
Li X, Gadzinsky A, Gong L, Tong H, Calderon V, Li Y, Kitamura D, Klein U, Langdon WY, Hou F, Zou YR, Gu H
Immunity 2018 Mar 20;48(3):530-541.e6
Immunity 2018 Mar 20;48(3):530-541.e6
Autophagy orchestrates the regulatory program of tumor-associated myeloid-derived suppressor cells.
Alissafi T, Hatzioannou A, Mintzas K, Barouni RM, Banos A, Sormendi S, Polyzos A, Xilouri M, Wielockx B, Gogas H, Verginis P
The Journal of clinical investigation 2018 Aug 31;128(9):3840-3852
The Journal of clinical investigation 2018 Aug 31;128(9):3840-3852
PTEN drives Th17 cell differentiation by preventing IL-2 production.
Kim HS, Jang SW, Lee W, Kim K, Sohn H, Hwang SS, Lee GR
The Journal of experimental medicine 2017 Nov 6;214(11):3381-3398
The Journal of experimental medicine 2017 Nov 6;214(11):3381-3398
Dichotomous Expression of TNF Superfamily Ligands on Antigen-Presenting Cells Controls Post-priming Anti-viral CD4(+) T Cell Immunity.
Chang YH, Wang KC, Chu KL, Clouthier DL, Tran AT, Torres Perez MS, Zhou AC, Abdul-Sater AA, Watts TH
Immunity 2017 Nov 21;47(5):943-958.e9
Immunity 2017 Nov 21;47(5):943-958.e9
Intestinal Epithelial and Intraepithelial T Cell Crosstalk Mediates a Dynamic Response to Infection.
Hoytema van Konijnenburg DP, Reis BS, Pedicord VA, Farache J, Victora GD, Mucida D
Cell 2017 Nov 2;171(4):783-794.e13
Cell 2017 Nov 2;171(4):783-794.e13
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Intracellular staining of freshly-harvested (left), unstimulated (middle), or 30-minute LPS-stimulated (right) normal human peripheral blood cells with Anti-Human CD14 FITC (Product # 11-0149-42) and Anti-Human phospho-S6 (S235/S236) PE, using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte/monocyte gate were used for analysis.
- Conjugate
- Yellow dye
