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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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Validation data
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- Product number
- 102-PA18AG - Provider product page
- Provider
- ReliaTech GmbH
- Product name
- VEGFR-2/KDR
- Antibody type
- Polyclonal
- Antigen
- recombinant human soluble KDR (D1-7)
- Description
- antibody affinity purified from serum
- Reactivity
- Human
- Host
- Rabbit
- Antigen sequence
ASVGLPSVSLDLPRLSIQKDILTIKANTTLQITCR
GQRDLDWLWPNNQSGSEQRVEVTECSDGLFCKTLT
IPKVIGNDTGAYKCFYRETDLASVIYVYVQDYRSP
FIASVSDQHGVVYITENKNKTVVIPCLGSISNLNV
SLCARYPEKRFVPDGNRISWDSKKGFTIPSYMISY
AGMVFCEAKINDESYQSIMYIVVVVGYRIYDVVLS
PSHGIELSVGEKLVLNCTARTELNVGIDFNWEYPS
SKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGVTR
SDQGLYTCAASSGLMTKKNSTFVRVHEKPFVAFGS
GMESLVEATVGERVRIPAKYLGYPPPEIKWYKNGI
PLESNHTIKAGHVLTIMEVSERDTGNYTVILTNPI
SKEKQSHVVSLVVYVPPQIGEKSLISPVDSYQYGT
TQTLTCTVYAIPPPHHIHWYWQLEEECANEPSQAV
SVTNPYPCEEWRSVEDFQGGNKIEVNKNQFALIEG
KNKTVSTLVIQAANVSALYKCEAVNKVGRGERVIS
FHVTRGPEITLQPDMQPTEQESVSLWCTADRSTFE
NLTWYKLGPQPLPIHVGELPTPVCKNLDTLWKLNA
TMFSNSTNDILIMELKNASLQDQGDYVCLAQDRKT
KKRHCVVRQLTVLERVAPTITGNLENQTTSIGESI
EVSCTASGNPPPQIMWFKDNETLVEDSGIVLKDGN
RNLTIRRVRKEDEGLYTCQACSVLGCAKVEAFFII
EGA- Antibody clone number
- Rabbit IG
- Vial size
- 50 µl
- Storage
- Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
- Handling
- Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.
Submitted references Lack of Evidence for the Direct Activation of Endothelial Cells by Adult Female and Microfilarial Excretory-Secretory Products
Weinkopff T, Lammie P, Diemert D
PLoS ONE 2011 August;6(8)
PLoS ONE 2011 August;6(8)
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Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- Western Analysis of anti-human VEGFR-2/KDR. Sample were loaded in 10% SDS-polyacrylamide gel under reducing conditions.
- Sample type
- Purified recombinant proteins
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- IHC with paraffin sections of human Glioblastoma tissue with a polyclonal rabbit anti-human VEGFR-2/KDR antibody [5 µg/ml]. The sections were pretreated with 2 mMol HCL in the microwave. The secondary goat anti-rabbit antibody was used 1:500 [Dianova]. Blocking was performed with 10% goat serum. The experiment was performed by Brigitte Maruschak, Dept. of Neuropathology, University Hospital Göttingen, Germany.
- Sample type
- human Glioblastom section
Supportive validation
- Submitted by
- ReliaTech GmbH (provider)
- Main image
- Experimental details
- FACS analysis with primary human umbilical vein endothelial cells (HUVEC).
