MAB3571-100

antibody from R&D Systems

Targeting: KDR CD309, FLK1, VEGFR, VEGFR2

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3571-100

Provider

R&D Systems

Product name

Human VEGFR2/KDR/Flk-1 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human VEGFR2 in direct ELISAs and Western blots. In Western blots, this antibody does not cross-react with recombinant human (rh) VEGFR1, rhVEGFR3, or recombinant mouse VEGFR2.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P35968


Isotype

IgG

Antibody clone number

89115

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Correlation of Vascular Endothelial Growth Factor subtypes and their receptors with melanoma progression: A next-generation Tissue Microarray (ngTMA) automated analysis.

Seyed Jafari SM, Wiedmer C, Cazzaniga S, Frangež Ž, Shafighi M, Beltraminelli H, Weber B, Simon HU, Hunger RE
PloS one 2018;13(11):e0207019

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Enhanced rapamycin delivery to hemangiomas by lipid polymer nanoparticles coupled with anti-VEGFR antibody.

Li H, Teng Y, Xu X, Liu J
International journal of molecular medicine 2018 Jun;41(6):3586-3596

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Delivery of sodium morrhuate to hemangioma endothelial cells using immunoliposomes conjugated with anti-VEGFR2/KDR antibody.

Li X, Ren X, Liang J, Ma W, Wang Z, Yang Z
International journal of nanomedicine 2017;12:6963-6972

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Analysis of angiogenesis related factors in glioblastoma, peritumoral tissue and their derived cancer stem cells.

D'Alessio A, Proietti G, Lama G, Biamonte F, Lauriola L, Moscato U, Vescovi A, Mangiola A, Angelucci C, Sica G
Oncotarget 2016 Nov 29;7(48):78541-78556

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Endothelial progenitor cells from human fetal aorta cure diabetic foot in a rat model.

Zhao WN, Xu SQ, Liang JF, Peng L, Liu HL, Wang Z, Fang Q, Wang M, Yin WQ, Zhang WJ, Lou JN
Metabolism: clinical and experimental 2016 Dec;65(12):1755-1767

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A novel serum-free monolayer culture for orderly hematopoietic differentiation of human pluripotent cells via mesodermal progenitors.

Niwa A, Heike T, Umeda K, Oshima K, Kato I, Sakai H, Suemori H, Nakahata T, Saito MK
PloS one 2011;6(7):e22261

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Tumour vascularization via endothelial differentiation of glioblastoma stem-like cells.

Ricci-Vitiani L, Pallini R, Biffoni M, Todaro M, Invernici G, Cenci T, Maira G, Parati EA, Stassi G, Larocca LM, De Maria R
Nature 2010 Dec 9;468(7325):824-8

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Development of anti-atherosclerotic tissue-engineered blood vessel by A20-regulated endothelial progenitor cells seeding decellularized vascular matrix.

Zhu C, Ying D, Mi J, Li L, Zeng W, Hou C, Sun J, Yuan W, Wen C, Zhang W
Biomaterials 2008 Jun;29(17):2628-36

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Adenoviral expression of vascular endothelial growth factor splice variants differentially regulate bone marrow-derived mesenchymal stem cells.

Lin H, Shabbir A, Molnar M, Yang J, Marion S, Canty JM Jr, Lee T
Journal of cellular physiology 2008 Aug;216(2):458-68

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Thrombopoietin enhances generation of CD34+ cells from human embryonic stem cells.

Srivastava AS, Nedelcu E, Esmaeli-Azad B, Mishra R, Carrier E
Stem cells (Dayton, Ohio) 2007 Jun;25(6):1456-61

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Isolation of renal progenitor cells from adult human kidney.

Bussolati B, Bruno S, Grange C, Buttiglieri S, Deregibus MC, Cantino D, Camussi G
The American journal of pathology 2005 Feb;166(2):545-55

Immunocytochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

VEGFR2/KDR/Flk-1 in HUVECs and Human HeLa Cell Line. VEGFR2/KDR/Flk-1 was detected in immersion fixed human umbilical vein endothelial cells (HUVECs; left panel) but is not detected in Hela human cervical epithelial carcinoma cell line (right panel) using Mouse Anti-Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3571) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm of HUVECs. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

VEGFR2/KDR/Flk-1 in Human Kidney. VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human kidney array using Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3571) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

VEGFR2/KDR/Flk-1 in Human Placenta. VEGFR2/KDR/Flk-1 was detected in immersion fixed paraffin-embedded sections of human placenta using 25 µg/mL Human VEGFR2/KDR/Flk-1 Monoclonal Antibody (Catalog # MAB3571) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.