NBP2-36760

antibody from Novus Biologicals

Targeting: EZR VIL2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-36760

Provider

Novus Biologicals

Product name

Mouse Monoclonal Ezrin Antibody

Antibody type

Monoclonal

Description

Protein A purified. Specificity of human Ezrin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.

Reactivity

Human

Host

Mouse

Antigen sequence

LSEQIQRALQLEEER


Isotype

IgG

Vial size

0.1 ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Ezrin Antibody (CL2384) [NBP2-36760] - Lane 1: Marker [kDa]. Lane 2: Human cell line U-251 MG

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Ezrin Antibody (CL2384) [NBP2-36760] - Analysis of extracts from U-251 cells, transfected with either control siRNA (siRNA ctrl) or target specific siRNA probes (siRNA#1, siRNA#2), using Anti-EZR monoclonal antibody. Downregulation of antibody signal confirms target specificity. Remaining % intensity, relative control lane, is indicated. Anti-GAPDH monoclonal antibody was used as loading control.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human small intestine shows strong positivity in apical membranes in glandular cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human placenta shows strong positivity in apical membrane in trophoblastic cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human tonsil shows strong membranous positivity in germinal center cells.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining of human skeletal muscle shows no positivity in striated muscle fibers as expected.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Ezrin Antibody (CL2384) [NBP2-36760] - Staining in human small intestine and skeletal muscle tissues. Corresponding EZR RNA-seq data are presented for the same tissues.