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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [2]
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- Product number
- PA5-36816 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Phospho-FOXO3A (Ser253) Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody detects endogenous protein at a molecular weight of 97 kDa.
- Concentration
- 1 mg/mL
Submitted references Airborne Particulate Matter (PM(10)) Inhibits Apoptosis through PI3K/AKT/FoxO3a Pathway in Lung Epithelial Cells: The Role of a Second Oxidant Stimulus.
García-Cuellar CM, Chirino YI, Morales-Bárcenas R, Soto-Reyes E, Quintana-Belmares R, Santibáñez-Andrade M, Sánchez-Pérez Y
International journal of molecular sciences 2020 Jan 11;21(2)
International journal of molecular sciences 2020 Jan 11;21(2)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Phospho-FOXO3A pSer253 in extracts from Raw264.7 cells using Phospho-FOXO3A pSer253 polyclonal antibody (Product # PA5-36816).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Phospho-FOXO3A pSer253 in paraffin-embedded human breast carcinoma using Phospho-FOXO3A pSer253 polyclonal antibody (Product # PA5-36816) at a dilution of 1:100.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Dose-response curve of the LY294002 inhibitor over FoxO3a. The cells were incubated to a concentration of LY294002 inhibitor (0.0, 0.5, 1, 2, 5, 10, 25, 50 uM) for 24 h. Cells were cultured for 24 h in F12K medium supplemented with 10% of fetal bovine serum (FBS); Control: cells unexposed; DMSO: cells exposed only to DMSO (vehicle for LY294002). ( A ) Representative image of western blot of pFoxO3 Ser253 , FoxO3, and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as housekeeping control and ( B ) data from a duplicated assay.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 4 Representative blot of ( A ) pFoxO3a Ser253 and total FoxO3a and ( B ) densitometry of levels using ImageJ software. Lung epithelial cells were pre-exposed to PM 10 (10 ug/cm 2 ) for 24 h, and then cells were treated with H 2 O 2 (500 mM) for 24 h. In lane 3 and 6 of the blot (panel A) are cells treated with LY294002 inhibitor (50 muM) for 1 h before the H 2 O 2 (500 uM) treatment. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) protein was used as loading control for western blot. * p < 0.01 versus control; ** p < 0.001 versus PM 10 + H 2 O 2 . The image is representative of three independent experiments, and values are the mean +- SD of three independent experiments.
