GTX13590

antibody from GeneTex

Targeting: PTEN BZS, MHAM, MMAC1, PTEN1, TEP1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX13590

Provider

GeneTex

Proper citation

GeneTex Cat#GTX13590, RRID:AB_368390

Product name

PTEN (phospho Ser385) antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Lysates prepared from HEK293 cells transiently transfected with WT PTEN (1, 3-6) or mutant S385A (2) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were either left untreated (1-5) or treated with lambda phosphatase (6), blocked with a 3% Milk-TBST buffer for one hour at room temperature, and incubated with PTEN [pS385] antibody for two hours at room temperature in a 3% Milk-TBST buffer, following prior incubation with: no peptide (1, 2, 6), the non-phosphopeptide corresponding to the immunogen (3), a generic phosphoserine-containing peptide (4), or, the phosphopeptide immunogen (5). After washing, membranes were incubated with goat F(ab)2 anti-rabbit IgG HRP conjugate and bands were detected. The data show that only the peptide corresponding to PTEN [pS385] blocks the antibody signal. Moreover, the signal is lost in the S385A single mutant. The data also show that phosphatase stripping eliminates the signal, verifying that the antibody is phospho-specific.