Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- GTX16125 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX16125, RRID:AB_422301
- Product name
- PTEN antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Sheep
- Storage
- Keep as concentrated solution. Store at 4°C short term. For extended storage aliquot and store at -20°C or below. Avoid freeze-thaw cycles.
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Purified sheep polyclonal antibody against pTEN used at 20 μg/ml. The PTEN status in these cell lines is as follows: Hec1A and KLE have wild-type PTEN, and AN3CA, MDA, and RL-95 have mutant PTEN (truncating mutations) 293T cells are supposed to have wild-type PTEN by sequence, but the levels are much lower than Hec1A or KLE. The lane marked 7 is 293T-PTEN is from 293T cells transfected with a FLAG-tagged PTEN. The FLAG-tagged proteins run slightly slower. Lane 1: ANA3CA Lane 2: Hec 1A Lane 3: KLE Lane 4: MDA Lane 5: RL- 95 Lane 6: -293T Lane 7: 292T cells transfected with FLAG pTEN Lane 8: negative control