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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- AP15248PU-N - Provider product page
- Provider
- OriGene
- Product name
- PTEN (N-term) rabbit polyclonal antibody, Purified
- Antibody type
- Polyclonal
- Description
- PTEN (N-term) rabbit polyclonal antibody, Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- PTEN
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of PTEN (arrow) using rabbit PTEN Antibody (N-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PTEN gene (Lane 2) (Origene Technologies).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis in HL-60 cell line lysates (35ug/lane) using PTEN Antibody (N-term) ?. This demonstrates the PTEN antibody detected the PTEN protein (arrow).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Confocal immunofluorescent analysis of PTEN Antibody (N-term) ?with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with PTEN antibody (N-term) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
- Validation comment
- IHC
