46-3079-41

antibody from Invitrogen Antibodies
Targeting: FCRL4 CD307d, FCRH4, IGFP2, IRTA1
Provider product page for 46-3079-41

Antibody data

Product number
46-3079-41 - Provider product page
Provider
Invitrogen Antibodies
Product name
Anti-CD307d (FcRL4) Monoclonal Antibody (413D12), PerCP-eFluor 710, eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The 413D12 monoclonal antibody reacts with human CD307d, which is also known as FcRL4, Fc receptor-like 4, FcRH4, IRTA4, or IGFP2. CD307d is an inhibitory receptor that is highly expressed on a subset of memory B cells resident in epithelial tissues and marginal zones of mucosal lymphoid tissues such as tonsils. CD307d has been shown to recruit the phosphatase SHP-1, thereby acting as an inhibitory receptor in B cells. CD307d is homologous to FcgammaRI and specifically binds IgA. To date, CD307d is the only known inhibitory receptor for IgA. Expression of CD307d is increased on memory B cells from patients with chronic infections such as HIV, suggesting that enhanced expression of inhibitory receptors, such as CD307d, may contribute to the inefficiency of antibody responses against HIV. Applications Reported: This 413D12 antibody has been reported for use in flow cytometric analysis. Applications Tested: This 413D12 antibody has been pre-titrated and tested by flow cytometric analysis of human tonsil tissue or transfected 293T cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
413D12
Vial size
25 Tests
Concentration
5 µL/Test
Storage
4° C, store in dark, DO NOT FREEZE!
FCRL4 protein structure - 46-3079-41 shown in red.