AF2316

antibody from Novus Biologicals

Targeting: PDGFRB CD140b, JTK12, PDGFR, PDGFR1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

AF2316

Provider

Novus Biologicals

Product name

Rabbit Polyclonal PDGF R beta Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human PDGF R beta when phosphorylated at Y1021 in Western blots.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human Phospho-PDGF R beta (Y1021) by Western Blot. Western blot shows lysates of human foreskin fibroblast untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-AA, PDGF-AB, and PDGF-BB (Catalog # 221-AA, 222-AB, and 220-BB, respectively) for 10 minutes. PVDF membrane was probed with 0.5 µg/mL of Rabbit Anti-Human Phospho-PDGF R beta (Y1021) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2316), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-PDGF R beta (Y1021) at approximately 185 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.