MAB9027

antibody from Novus Biologicals

Targeting: PDGFRB CD140b, JTK12, PDGFR, PDGFR1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Antibody Data

Product number

MAB9027

Provider

Novus Biologicals

Product name

Rabbit Monoclonal PDGF R beta Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from cell culture supernatant. Detects human and mouse PDGF R beta when phosphorylated at Y751 in Western blots.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Assembly of fibronectin fibrils selectively attenuates platelet-derived growth factor-induced intracellular calcium release in fibroblasts.

Farrar CS, Hocking DC
The Journal of biological chemistry 2018 Nov 30;293(48):18655-18666

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse Phospho-PDGF R beta (Y751) by Western Blot. Western blot shows lysates of CCD-1070Sk human foreskin fibroblast cell line and NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 20 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse Phospho-PDGF R beta (Y751) Monoclonal Antibody (Catalog # MAB9027) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for PDGF R beta at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Mouse Phospho-PDGF R beta (Y751) by Simple Western^TM. Simple Western lane view shows lysates of NIH-3T3 mouse embryonic fibroblast cell line untreated (-) or treated (+) with 100 ng/mL Recombinant Human PDGF-BB (Catalog # 220-BB) for 20 minutes, loaded at 0.2 mg/mL. A specific band was detected for PDGF R beta at approximately 240 kDa (as indicated) using 1 µg/mL of Rabbit Anti-Human/Mouse Phospho-PDGF R beta (Y751) Monoclonal Antibody (Catalog # MAB9027). This experiment was conducted under reducing conditions and using the 66-440 kDa separation system.