22590002-0.1mg

antibody from Novus Biologicals

Targeting: ERBB3 HER3, LCCS2

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Antibody Data

Product number

22590002-0.1mg

Provider

Novus Biologicals

Product name

Rabbit Polyclonal ErbB3/Her3 Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. This product is specific for Human ErbB 3.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

0.1 mg

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: ErbB3/Her3 Antibody [22590002] - Western Blot was performed using affinity purified SEQer ERBB3 antibody, aa(146-245) antibody. The lanes contain 5-30ug of a whole cell extract. Final concentration of antibodies = 0.1ug/ml (1:10,000 dilution). The blot was probed overnight with the SEQer ERBB3 antibody, aa(146-245) antibody. Blot was then washed according to protocol and probed with goat-anti-Rabbit-HRP conjugate at 1:5000 dilution, washed and developed using chemiluminescence (film exposure 5-30sec). The protein was detected as represented by the band shown. The detected protein band is estimated at 110 KDa, and the predicated molecular weight of ERBB3 is 140 KDa. The difference of molecular weight between empirical and predicted results are unclear. It could cause by post-translational modification, isotype, proteolytic degradation, gel artifacts or the other reasons.