Antibody data
- Antibody Data
- Antigen structure
- References [4]
- Comments [0]
- Validations
- Flow cytometry [2]
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Validation data
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- Product number
- 46-6555-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ErbB3 (HER-3) Monoclonal Antibody (SGP1), PerCP-eFluor™ 710, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: This SGP1 monoclonal antibody reacts with human c-ErbB3, which is also known as HER3. This epidermal growth factor (EGF) receptor family member is expressed on normal human tissues, particularly on neurons and cells of the gastrointestinal tract. c-ErbB3 is overexpressed in a variety of cancers, including breast, ovary, and gastrointestinal carcinomas. Upon binding its ligand heregulin beta-1, c-ErbB3 is activated by heterodimerization with ErbB2 or EGFR1. Dependent on PI3K signaling, c-ErbB3 mediates tumor cell invasion, intravasation, and metastasis. Expression of this cell surface receptor has been used as a prognostic marker for breast cancer.
- Antibody clone number
- SGP1
- Concentration
- 5 µL/Test
Submitted references Structure-based mutagenesis of the substrate-recognition domain of Nrdp1/FLRF identifies the binding site for the receptor tyrosine kinase ErbB3.
c-erbB-3: a nuclear protein in mammary epithelial cells.
Expression of c-erbB3 protein in primary breast carcinomas.
A monoclonal antibody to the human c-erbB3 protein stimulates the anchorage-independent growth of breast cancer cell lines.
Bouyain S, Leahy DJ
Protein science : a publication of the Protein Society 2007 Apr;16(4):654-61
Protein science : a publication of the Protein Society 2007 Apr;16(4):654-61
c-erbB-3: a nuclear protein in mammary epithelial cells.
Offterdinger M, Schöfer C, Weipoltshammer K, Grunt TW
The Journal of cell biology 2002 Jun 10;157(6):929-39
The Journal of cell biology 2002 Jun 10;157(6):929-39
Expression of c-erbB3 protein in primary breast carcinomas.
Naidu R, Yadav M, Nair S, Kutty MK
British journal of cancer 1998 Nov;78(10):1385-90
British journal of cancer 1998 Nov;78(10):1385-90
A monoclonal antibody to the human c-erbB3 protein stimulates the anchorage-independent growth of breast cancer cell lines.
Rajkumar T, Gullick WJ
British journal of cancer 1994 Sep;70(3):459-65
British journal of cancer 1994 Sep;70(3):459-65
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of the MCF-7 cell line with Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (blue histogram) or Anti-Human c-ErbB3/HER3 PerCP-eFluor® 710 (purple histogram). Total viable cells were used for analysis.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockout of ErbB3 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR764358_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of ErbB3 was performed by staining T-47D ErbB3 Knock out cells with0.125 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-eFluor™ 710, eBioscience™ (Product # 46-4714-82, yellow histogram) or0.125 µgErbB3 (HER-3) Monoclonal Antibody (SGP1), PerCP-eFluor™ 710, eBioscience™ (Product # 46-6555-42, blue histogram).T47D Cas9 control cells were also stained with0.125 µgErbB3 (HER-3) Monoclonal Antibody (SGP1), PerCP-eFluor™ 710, eBioscience™ (Product # 46-6555-42, pink histogram). Lossof signal was observed in the ErbB3 KOcells stained with ErbB3 antibody clone SGP1 but not in the control Cas9cells. Viable cells were used for analysis, as determined by Fixable Viability Dye eFluor™ 450 (Product # 65-0863-14).