36-0200

antibody from Invitrogen Antibodies

Targeting: FLT4 PCL, VEGFR-3, VEGFR3

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

36-0200

Provider

Invitrogen Antibodies

Product name

Anti-VEGF Receptor 3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

0.25 mg/ml

Storage

-20°C

References

Submitted references

Expression of factors involved in the regulation of angiogenesis in the full-term human placenta: Effects of in vitro fertilization.

Li C, Zhang Y, Tang L, Zhao H, Gao C, Gao L, Cui Y, Liu J
Reproductive biology 2016 Jun;16(2):104-12

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Immunohistochemical expression and distribution of VEGFR-3 in malignant mesothelioma.

Filho AL, Baltazar F, Bedrossian C, Michael C, Schmitt FC
Diagnostic cytopathology 2007 Dec;35(12):786-91

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of breast carcinoma tissue using Rb anti-VEGF Receptor-3 (C-term) (PAD: ZMD.251) (Product # 36-0200).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of FLT-4/VEGFR3 showing staining in the cytoplasm of paraffin-embedded human placenta tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Anti- FLT-4/VEGFR3 Polyclonal Antibody (Product # 36-0200) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.