103-M36

antibody from ReliaTech GmbH

Targeting: FLT4 PCL, VEGFR-3, VEGFR3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

103-M36

Provider

ReliaTech GmbH

Product name

VEGFR-3/FLT-4

Antibody type

Monoclonal

Description

antibody purified from hybridoma supernatant

Reactivity

Mouse

Host

Rat

Antigen sequence

DPSGYSMTPPTLNITEDSYVIDTGDSLSISCRGQH
PLEWTWPGAQEVLTTGGKDSEDTRVVHDCEGTEAR
PYCKVLLLAQTHANNTGSYHCYYKYIKARIEGTTA
ASTYVFVRDFKHPFINKPDTLLVNRKDSMWVPCLV
SIPGLNITLRSQSSALHPDGQEVLWDDRRGMRVPT
QLLRDALYLQCETTWGDQNFLSNLFVVHITGNELY
DIQLYPKKSMELLVGEKLVLNCTVWAEFDSGVTFD
WDYPGKQAERAKWVPERRSQQTHTELSSILTIHNV
SQNDLGPYVCEANNGIQRFRESTEVIVHEKPFISV
EWLKGPVLEATAGDELVKLPVKLAAYPPPEFQWYK
DRKAVTGRHNPHALVLKEVTEASAGVYTLALWNSA
AGLRQNISLELVVNVPPHIHEKEASSPSIYSRHSR
QTLTCTAYGVPQPLSVQWHWRPWTPCKTFAQRSLR
RRQQRDGMPQCRDWKEVTTQDAVNPIESLDSWTEF
VEGKNKTVSKLVIQDANVSAMYKCVVVNKVGQDER
LIYFYVTTIPDGFSIESEPSEDPLEGQSVRLSCRA
DNYTYEHLRWYRLNLSTLHDAQGNPLLLDCKNVHL
FATPLEANLEEAEPGARHATLSLNIPRVAPEDEGD
YVCEVQDRRSQDKHCHKKYLSVQALEAPRLTQNLT
DLLVNVSDSLEMRCPVAGAHVPSIVWYKDERLLEK
ESGIDLADSNQRLSIQRVREEDAGRYLCSVCNAKG
CVNSSASVAVEGSEDKGSMEHHHHHH


Antibody clone number

(#7A3)

Storage

Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.

Handling

Restore in sterile water to a concentration of 0.1-1.0 mg/ml. The antibody solution should be gently mixed before use.

References

Submitted references

Mouse lung contains endothelial progenitors with high capacity to form blood and lymphatic vessels

Schniedermann J, Rennecke M, Buttler K, Richter G, Städtler A, Norgall S, Badar M, Barleon B, May T, Wilting J, Weich H
BMC Cell Biology 2010 ;11(1):50

Western blot

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

Western analysis with VEGFR-3/FLT-4 expressing PAEC endothelial cells. Total lysate of both cell types were subjected to SDS-PAGE and subsequent Western analysis with the monoclonal (#103-M36) antibody.

Sample type

cell lysate

Immunocytochemistry

Supportive validation

Submitted by

ReliaTech GmbH (provider)

Main image

Experimental details

Immunofluorescence staining (green) of VEGFR-3/FLT-4 in primary mouse endothelial cells (SnoMec) with anti-mouse VEGFR-3/FLT-4 #7A3 (10µg/ml) and counter staining of nuclei with Dapi. As secondary antibody goat anti-mouse ALEXA Flour 488 (Dianova) was used 1:400.