AF800

antibody from Novus Biologicals

Targeting: BCL2L1 Bcl-X, bcl-xL, bcl-xS, BCL2L, BCLX, PPP1R52

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

AF800

Provider

Novus Biologicals

Product name

Rabbit Polyclonal bcl-x Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified. Detects human and mouse Bcl-x in Western blots.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

50 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Nuclear colocalization and interaction between bcl-xL and cdk1(cdc2) during G2/M cell-cycle checkpoint.

Schmitt E, Beauchemin M, Bertrand R
Oncogene 2007 Aug 30;26(40):5851-65

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Type I collagen promotes the malignant phenotype of pancreatic ductal adenocarcinoma.

Armstrong T, Packham G, Murphy LB, Bateman AC, Conti JA, Fine DR, Johnson CD, Benyon RC, Iredale JP
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Nov 1;10(21):7427-37

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Antisense oligonucleotide targeting of Raf-1: importance of raf-1 mRNA expression levels and raf-1-dependent signaling in determining growth response in ovarian cancer.

Mullen P, McPhillips F, MacLeod K, Monia B, Smyth JF, Langdon SP
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Mar 15;10(6):2100-8

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Antisense oligonucleotide targeting of Raf-1: importance of raf-1 mRNA expression levels and raf-1-dependent signaling in determining growth response in ovarian cancer.

Mullen P, McPhillips F, MacLeod K, Monia B, Smyth JF, Langdon SP
Clinical cancer research : an official journal of the American Association for Cancer Research 2004 Mar 15;10(6):2100-8

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The anti-apoptotic effect of Notch-1 requires p56lck-dependent, Akt/PKB-mediated signaling in T cells.

Sade H, Krishna S, Sarin A
The Journal of biological chemistry 2004 Jan 23;279(4):2937-44

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Ligation of CD27 on B cells in vivo during primary immunization enhances commitment to memory B cell responses.

Raman VS, Akondy RS, Rath S, Bal V, George A
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 1;171(11):5876-81

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Ligation of CD27 on B cells in vivo during primary immunization enhances commitment to memory B cell responses.

Raman VS, Akondy RS, Rath S, Bal V, George A
Journal of immunology (Baltimore, Md. : 1950) 2003 Dec 1;171(11):5876-81

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T cell costimulation through CD28 depends on induction of the Bcl-xgamma isoform: analysis of Bcl-xgamma-deficient mice.

Ye Q, Press B, Kissler S, Yang XF, Lu L, Bassing CH, Sleckman BP, Jansson M, Panoutsakopoulou V, Trimble LA, Alt FW, Cantor H
The Journal of experimental medicine 2002 Jul 1;196(1):87-95

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T cell costimulation through CD28 depends on induction of the Bcl-xgamma isoform: analysis of Bcl-xgamma-deficient mice.

Ye Q, Press B, Kissler S, Yang XF, Lu L, Bassing CH, Sleckman BP, Jansson M, Panoutsakopoulou V, Trimble LA, Alt FW, Cantor H
The Journal of experimental medicine 2002 Jul 1;196(1):87-95

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse Bcl-x by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line (5 x 10⁴ cells, lane 1 and 2.5 x 10⁴ cells, lane 2) and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 0.3 µg/mL of Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Bcl-x at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human and Mouse Bcl-x by Simple Western^TM. Simple Western lane view shows lysates of A431 human epithelial carcinoma cell line and NIH-3T3 mouse embryonic fibroblast cell line, loaded at 0.2 mg/mL. A specific band was detected for Bcl-x at approximately 34 kDa (as indicated) using 3 µg/mL of Rabbit Anti-Human/Mouse Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunoprecipitation

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunoprecipitation of Human Bcl-x. Bcl-x was immunoprecipitated from lysates (7 x 10⁵ cells) of A431 human epithelial carcinoma cell line following incubation with 3 µg Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800) for 30-60 minutes at 4 °C. Bcl-x-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno-precipitated Bcl-x was detected by Western blot using 0.3 µg/mL Rabbit Anti-Human Bcl-x Antigen Affinity-purified Polyclonal Antibody (Catalog # AF800). View our recommended buffer recipes for immunoprecipitation.