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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [2]
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- Product number
- NBP2-33124 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal beta-Catenin Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified. Beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. In normal tissues, beta-catenin is localized to the membrane of epithelial cells, consistent with its role in the cell adhesion complex. In breast ductal neoplasia, beta-catenin is usually localized in cellular membranes. However, in lobular neoplasia, a marked redistribution of beta-catenin throughout the cytoplasm results in a diffuse cytoplasmic pattern. Immuno-staining of beta-catenin and E-cadherin is helps in the accurate identification of ductal and lobular neoplasms, including a distinction between low-grade ductal carcinoma in situ (DCIS) and lobular carcinoma. Additionally, some rectal and gastric adenocarcinomas demonstrate diffuse cytoplasmic beta-catenin staining and a lack of membranous staining, mimicking the staining pattern observed with lobular breast carcinomas.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: beta-Catenin Antibody - BSA Free [NBP2-33124] - Formalin-paraffin breast lobular carcinoma stained with catenin, beta PAb. Note cytoplasmic staining in lobular carcinoma.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: beta-Catenin Antibody - BSA Free [NBP2-33124] - Formalin-fixed, paraffin-embedded human breast ductal carcinoma stained with beta- Catenin antibody (1:100), peroxidase-conjugate and Warp Red chromogen (20X). Note specific membrane staining in ductal carcinoma.
