OSG00014W

antibody from Invitrogen Antibodies

Targeting: FGF2 FGFB

Western blot (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

OSG00014W

Provider

Invitrogen Antibodies

Product name

FGF2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Glycerol (1:1) may be added for added stability.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Fibroblast Growth Factor (FGF2) in the equine placenta. Tissue was formalin fixed and paraffin embedded. Staining was performed in Bond-MAX automated system following manufacturer’s protocols. Briefly, automated dewaxing and rehydration step, followed by 100°C for 10 min antigen retrieval using an EDTA based retrieval solution (pH of 8.2). Post-retrieval, slides were incubated with 3% hydrogen peroxide (5 minutes), diluted primary antibody (30 minutes), blocking reagent (8 minutes), horseradish peroxidase-labeled polymer (8 minutes), and diaminobenzidine substrate (10 minutes). Primary antibody FGF2 (Product # OSG00014W) was diluted 1:200. Counterstain was hematoxylin. Sections were dehydrated and mounted with a coverslip. Images were taken at 100X magnification. Notably, FGF2 immunohistochemistry analysis produced heavy tissue staining. The negative control was assessed (omission of primary antibody), and no staining was observed (data not shown). Data courtesy of Antibody Data Exchange Program.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis performed on sections of human rectum using FGF2 Polyclonal Antibody (Product # OSG00014W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermos PT Module Detection was done using Novolink HRP polymer from Leica following manufacturers instruction Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis performed on sections of human rectum using FGF2 Polyclonal Antibody (Product # OSG00014W). HIER: 1 mM EDTA, pH 8 for 20 min using Thermos PT Module. Detection was done using Novolink HRP polymer from Leica following manufacturers instruction. Primary antibody: dilution 1:1000, incubated 30 min at RT. Sections were counterstained with Harris Hematoxylin.