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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Immunohistochemistry [2]
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- Product number
- BAF233 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal FGF basic/FGF2/bFGF Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human FGF basic/FGF2/bFGF in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) FGF acidic and rhFGF-7 is observed under non-reducing conditions.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Biotin
- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- FGF basic in Human Ovarian Cancer Tissue. FGF basic was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Human FGF basic Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF233) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- FGF basic in Human Ovary. FGF basic was detected in immersion fixed paraffin-embedded sections of human ovarian array using Human FGF basic Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF233) at 10 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
