Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [5]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP1-76614 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-76614, RRID:AB_11021647
- Product name
- Rabbit Polyclonal ACE-2 Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. Anti-ACE2 has no cross response to ACE1.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references The ACE2/Ang-(1-7)/Mas Axis Regulates the Development of Pancreatic Endocrine Cells in Mouse Embryos.
Wang L, Liang J, Leung PS
PloS one 2015;10(6):e0128216
PloS one 2015;10(6):e0128216
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACE-2 Antibody [NBP1-76614] - Human kidney lysate with ACE2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACE-2 Antibody [NBP1-76614] - Validation in Human Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACE-2 Antibody [NBP1-76614] - Loading: 15 ug of lysates per lane. Antibodies: ACE2, (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACE-2 Antibody [NBP1-76614] - Validation in Rat Skin Tissue Loading: 15 ug of lysates per lane. Antibodies: ACE2 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACE-2 Antibody [NBP1-76614] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues Loading: 15 ug of lysates per lane. Antibodies: ACE2, (2 ug/mL), ACE2, (2 ug/mL), ACE2, (2 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACE-2 Antibody [NBP1-76614] - Analysis of paraffin-embedded human kidney tissue using anti-ACE2 antibody 2 ug/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.