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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Flow cytometry [1]
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- Product number
- IC1427F - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal ADAM10 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human ADAM10 in direct ELISAs and Western blots. In Western blots, shows less than 5% cross-reactivity with recombinant human (rh) ADAM8, 9, 15, 17/TACE, and recombinant mouse ADAM10.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 degreesC as supplied.
Submitted references ADAM10-Interacting Tetraspanins Tspan5 and Tspan17 Regulate VE-Cadherin Expression and Promote T Lymphocyte Transmigration.
The impact of α-toxin on host cell plasma membrane permeability and cytokine expression during human blood infection by CA-MRSA USA300.
Reyat JS, Chimen M, Noy PJ, Szyroka J, Rainger GE, Tomlinson MG
Journal of immunology (Baltimore, Md. : 1950) 2017 Jul 15;199(2):666-676
Journal of immunology (Baltimore, Md. : 1950) 2017 Jul 15;199(2):666-676
The impact of α-toxin on host cell plasma membrane permeability and cytokine expression during human blood infection by CA-MRSA USA300.
Nygaard TK, Pallister KB, Zurek OW, Voyich JM
Journal of leukocyte biology 2013 Nov;94(5):971-9
Journal of leukocyte biology 2013 Nov;94(5):971-9
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of ADAM10 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ADAM10 Ectodomain Fluorescein-conjugated Monoclonal Antibody (Catalog # IC1427F, filled histogram) or isotype control antibody (Catalog # IC0041F, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
