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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Flow cytometry [1]
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- Product number
- IC1427A - Provider product page
- Provider
- R&D Systems
- Product name
- Human ADAM10 Ectodomain APC-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human ADAM10 in direct ELISAs and Western blots. In Western blots, shows less than 5% cross-reactivity with recombinant human (rh) ADAM8, 9, 15, 17/TACE, and recombinant mouse ADAM10.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Red dye
- Antigen sequence
O14672- Isotype
- IgG
- Antibody clone number
- 163003
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
Submitted references Nuclear trafficking of the HIV-1 pre-integration complex depends on the ADAM10 intracellular domain.
Endsley MA, Somasunderam AD, Li G, Oezguen N, Thiviyanathan V, Murray JL, Rubin DH, Hodge TW, O'Brien WA, Lewis B, Ferguson MR
Virology 2014 Apr;454-455:60-6
Virology 2014 Apr;454-455:60-6
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of ADAM10 in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ADAM10 Ectodomain APC-conjugated Monoclonal Antibody (Catalog # IC1427A, filled histogram) or isotype control antibody (Catalog # IC0041A, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
