Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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Validation data
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- Product number
- 710695 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Huntingtin Recombinant Polyclonal Antibody (3HCLC)
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This antibody is predicted to react with Monkey, Mouse and Bovine.
- Antibody clone number
- 3HCLC
- Concentration
- 0.5 mg/mL
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescence was performed on fixed and permeabilized SH-SY5Y cells for detection of HTT using HTT Recombinant Rabbit Polyclonal Antibody (Product # 710695, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of HTT protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of HTT. Panel e) shows loss of signal by competition with the HTT peptide, demonstrating antibody specificity and panel f) represents control cells with no primary antibody to assess background.