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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Product number
- ABIN401028 - Provider product page
- Provider
- antibodies-online
- Product name
- anti-Ubiquitin-Like Modifier Activating Enzyme 1 (UBA1) antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein corresponding to full length Human Ubiquitin Activating Enzyme E1 fused with GST
- Description
- Purified
- Reactivity
- Human, Mouse, Rat, Canine, Rabbit
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.5 mg
- Concentration
- 5.0 mg/mL (by UV absorbance at 280 nm)
- Storage
- Prior to reconstitution store at 2-8°C. Following reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at-20°C for longer.
- Handling
- Avoid repeated freezing and thawing.
Submitted references Screening of Hsp105alpha-binding proteins using yeast and bacterial two-hybrid systems.
Large-scale characterization of HeLa cell nuclear phosphoproteins.
A non-proteolytic role for ubiquitin in Tat-mediated transactivation of the HIV-1 promoter.
The ubiquitin-activating enzyme E1 is phosphorylated and localized to the nucleus in a cell cycle-dependent manner.
Ubiquitin-activating enzyme, E1, is phosphorylated in mammalian cells by the protein kinase Cdc2.
Phosphorylation of ubiquitin-activating enzyme in cultured cells.
Saito Y, Doi K, Yamagishi N, Ishihara K, Hatayama T
Biochemical and biophysical research communications 2004 Feb 6;314(2):396-402
Biochemical and biophysical research communications 2004 Feb 6;314(2):396-402
Large-scale characterization of HeLa cell nuclear phosphoproteins.
Beausoleil SA, Jedrychowski M, Schwartz D, Elias JE, Villén J, Li J, Cohn MA, Cantley LC, Gygi SP
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
Proceedings of the National Academy of Sciences of the United States of America 2004 Aug 17;101(33):12130-5
A non-proteolytic role for ubiquitin in Tat-mediated transactivation of the HIV-1 promoter.
Brès V, Kiernan RE, Linares LK, Chable-Bessia C, Plechakova O, Tréand C, Emiliani S, Peloponese JM, Jeang KT, Coux O, Scheffner M, Benkirane M
Nature cell biology 2003 Aug;5(8):754-61
Nature cell biology 2003 Aug;5(8):754-61
The ubiquitin-activating enzyme E1 is phosphorylated and localized to the nucleus in a cell cycle-dependent manner.
Stephen AG, Trausch-Azar JS, Ciechanover A, Schwartz AL
The Journal of biological chemistry 1996 Jun 28;271(26):15608-14
The Journal of biological chemistry 1996 Jun 28;271(26):15608-14
Ubiquitin-activating enzyme, E1, is phosphorylated in mammalian cells by the protein kinase Cdc2.
Nagai Y, Kaneda S, Nomura K, Yasuda H, Seno T, Yamao F
Journal of cell science 1995 Jun;108 ( Pt 6):2145-52
Journal of cell science 1995 Jun;108 ( Pt 6):2145-52
Phosphorylation of ubiquitin-activating enzyme in cultured cells.
Cook JC, Chock PB
Proceedings of the National Academy of Sciences of the United States of America 1995 Apr 11;92(8):3454-7
Proceedings of the National Academy of Sciences of the United States of America 1995 Apr 11;92(8):3454-7
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