V2198

antibody from NSJ Bioreagents

Targeting: MUC5AC MUC5

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

V2198

Provider

NSJ Bioreagents

Product name

MUC5AC Antibody Mucin-5AC

Antibody type

Monoclonal

Antigen

M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient was used as the immunogen for this MUC5AC antibody.

Description

Protein G purified antibody

Reactivity

Human, Mouse, Rat, Feline, Porcine, Rabbit, Simian

Host

Mouse

Conjugate

Unconjugated

Antibody clone number

45M1

Vial size

20 µg, 100 µg (with sodium azide), 100 µg (without sodium azide), 7 ml (prediluted for IHC use, if applicable)

Storage

Store the MUC5AC antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).

References

Submitted references

Luteolin inhibited the gene expression, production and secretion of MUC5AC mucin via regulation of nuclear factor kappa B signaling pathway in human airway epithelial cells.

Lee HJ, Seo HS, Ryu J, Yoon YP, Park SH, Lee CJ
Pulmonary pharmacology & therapeutics 2015 Apr;31:117-22

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Club cell 10-kDa protein attenuates airway mucus hypersecretion and inflammation.

Tokita E, Tanabe T, Asano K, Suzaki H, Rubin BK
The European respiratory journal 2014 Oct;44(4):1002-10

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Apigenin and Wogonin Regulate Epidermal Growth Factor Receptor Signaling Pathway Involved in MUC5AC Mucin Gene Expression and Production from Cultured Airway Epithelial Cells.

Sikder MA, Lee HJ, Ryu J, Park SH, Kim JO, Hong JH, Seok JH, Lee CJ
Tuberculosis and respiratory diseases 2014 Mar;76(3):120-6

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Immunomodulatory effect of linezolid on methicillin-resistant Staphylococcus aureus supernatant-induced MUC5AC overexpression in human airway epithelial cells.

Kaku N, Yanagihara K, Morinaga Y, Yamada K, Harada Y, Migiyama Y, Nagaoka K, Nakamura S, Izumikawa K, Kohno S
Antimicrobial agents and chemotherapy 2014 Jul;58(7):4131-7

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The ocular surface phenotype of Muc5ac and Muc5b null mice.

Marko CK, Tisdale AS, Spurr-Michaud S, Evans C, Gipson IK
Investigative ophthalmology & visual science 2014 Jan 15;55(1):291-300

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Azithromycin inhibits MUC5AC induction via multidrug-resistant Acinetobacter baumannii in human airway epithelial cells.

Yamada K, Morinaga Y, Yanagihara K, Kaku N, Harada Y, Uno N, Nakamura S, Imamura Y, Hasegawa H, Miyazaki T, Izumikawa K, Kakeya H, Mikamo H, Kohno S
Pulmonary pharmacology & therapeutics 2014 Aug;28(2):165-70

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Cigarette smoke alters non-neuronal cholinergic system components inducing MUC5AC production in the H292 cell line.

Montalbano AM, Albano GD, Anzalone G, Bonanno A, Riccobono L, Di Sano C, Gagliardo R, Siena L, Pieper MP, Gjomarkaj M, Profita M
European journal of pharmacology 2014 Aug 5;736:35-43

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of human stomach stained with MUC5AC antibody (45M1).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of human gastric carcinoma stained with MUC5AC antibody (45M1).

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of rat stomach stained with MUC5AC antibody (45M1).

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

FACS staining (intracellular) of A549 cells using MUC5AC antibody (red) and isotype control (green).