Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- MA1-26000 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- VAMP1 Monoclonal Antibody (4E206)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- MA1-26000 detects a band of ~38 kDa. Postive control of mouse and rat brain tissue extract suggested. No reactivity was detected in mouse liver extracts.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Mouse
- Isotype
- IgM
- Antibody clone number
- 4E206
- Vial size
- 50 µL
- Concentration
- 1.0 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of PC12 (Lane 1), tissue extracts of Mouse Brain (Lane 2), Mouse Liver (Lane 3) and Rat Brain (Lane 4). The blot was probed with Anti-Synaptophysin Monoclonal Antibody (4E206) (Product # MA1-26000, 1:2000 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 38 kDa band corresponding to synaptophysin was observed across all cell lines, mouse and rat tissue extracts tested.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of various samples using VAMP1 Monoclonal Antibody (4E206) (Product # MA1-26000). 1: MW marker. 2: Mouse brain extract. 3: Rat brain extract.