MA5-17621

antibody from Invitrogen Antibodies

Targeting: S100A8 60B8AG, CAGA, CFAG, CGLA, MRP8, P8

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17621

Provider

Invitrogen Antibodies

Product name

S100A8 Monoclonal Antibody (CF-145)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-17621 targets Calgranulin A in FACS applications and shows reactivity with Human samples.

Antibody clone number

CF-145

Concentration

1 mg/mL

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of S100A8 was performed using 70% confluent log phase SK-BR-3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with S100A8 Monoclonal Antibody (CF-145) (Product # MA5-17621) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing localization to nucleus and cytoplasm. Panel e shows A549 cells with no expression of S100A8. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of human whole blood (granulocyte population) stained using a Calgranulin A monoclonal antibody (Product # MA5-17621) (filled histogram) or mouse IgG2b isotype control (open histogram).