PA1-41004

antibody from Invitrogen Antibodies

Targeting: H2AX H2AFX

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Recommended by provider)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-41004

Provider

Invitrogen Antibodies

Product name

Histone H2A.X Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Suggested positive control: Jurkat or PBMC.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Skin models for cutaneous melioidosis reveal Burkholderia infection dynamics at wound's edge with inflammasome activation, keratinocyte extrusion and epidermal detachment.

Ku JWK, Marsh ST, Nai MH, Robinson KS, Teo DET, Zhong FL, Brown KA, Lim TC, Lim CT, Gan YH
Emerging microbes & infections 2021 Dec;10(1):2326-2339

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82-kDa choline acetyltransferase and SATB1 localize to β-amyloid induced matrix attachment regions.

Winick-Ng W, Caetano FA, Winick-Ng J, Morey TM, Heit B, Rylett RJ
Scientific reports 2016 Apr 7;6:23914

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), MDA-MB-231 (Lane 3) and COS-7 (Lane 4). The blot was probed with Anti-Histone H2A.X Polyclonal Antibody (Product # PA1-41004, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to H2A.X was observed across the cell lines tested.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Histone H2A.X in human PBMC lysate. Sample was incubated in Histone H2A.X polyclonal antibody (Product # PA1-41004).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on acid extracts (20 µg lysate) of HeLa (Lane 1), K-562 (Lane 2), MDA-MB-231 (Lane 3) and COS-7 (Lane 4). The blot was probed with Anti-Histone H2A.X Polyclonal Antibody (Product # PA1-41004, 1 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/mL, 1:4000 dilution). A 17 kDa band corresponding to H2A.X was observed across the cell lines tested.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Histone H2A.X in Human breast. Samples were incubated with Histone H2A.X polyclonal antibody (Product # PA1-41004) using a dilution of 5 µg/mL. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Enrichment of endogenous Histone 1F0 protein at specific gene loci using Anti-Histone 1F0 Antibody: Chromatin Immunoprecipitation (ChIP) was performed using Anti-Histone 1F0 Polyclonal Antibody (Product # PA5-51466, 3 µg) on sheared chromatin from 2 million U-2 OS cells using the "MAGnify ChIP system" kit (Product # 49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA was analyzed by qPCR with PCR primer pairs over the GAPDH gene (active) and MYOD, SAT2 satellite repeats, SAT Alpha (inactive). A schematic diagram of the GAPDH gene is shown on top of the figure. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.