MA5-16167

antibody from Invitrogen Antibodies

Targeting: TP73 P73

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA5-16167

Provider

Invitrogen Antibodies

Product name

p73 Monoclonal Antibody (5B429)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

5B429

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysisof p73 in A) transfectedcell lysate and B) HeLa cell lysate using a p73 monoclonal antibody (Product # MA5-16167) at 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of p73 in A) transfected cell lysate and B) HeLa cell lysate. Samples were incubated in p73 monoclonal antibody (Product # MA5-16167) using a dilution of 2 µg/mL.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of p73 in 1.0 mg/mL HeLa lysate. Samples were incubated in p73 monoclonal (Product # MA5-16167). This experiment was performed under reducing conditions using the 12-230 kDa separation system. * Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis was performed on modified whole cell extracts (1% SDS) (30 µg lysate) of HeLa (Lane 1), SK-Ov-3 (Lane 2), SK-BR-3 (Lane 3), MCF-7 (Lane 4) and MDA-MB-231 (Lane 5). The blot was probed with p73 Monoclonal Antibody (5B429) (Product # MA5-16167, 1µg/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25µg/ml, 1:4000 dilution). A 40kDa band corresponding to P73 was observed across the cell lines tested.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of p73 in immersion fixed Hela human cell line using. Samples were incubated in p73 monoclonal antibody (Product # MA5-16167) using a dilution of 3 µg/mL for 3 hours at room temperature followed by NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red) and counterstained with DAPI (blue). Staining was observed in the cytoplasm and in the nuclei.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of p73 in formalin-fixed paraffin-embedded human breast cancer tissue. Samples were incubated in p73 monoclonal antibody (Product # MA5-16167) using a dilution of 5 µg/mL. Staining of tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of p73 in Human HeLa Cell Line. Samples were incubated in p73 monoclonal antibody (Product # MA5-16167) or Mouse IgG1 isotype control followed by APC-conjugated Anti-Mouse IgG Secondary Antibody. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer and permeabilized with Flow Cytometry Permeabilization/Wash Buffer.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of p73 in HeLa cells. Samples were incubated in p73 monoclonal antibody (Product # MA5-16167) using a dilution of 1 µg/mL for 30 minutes followed by mouse F(ab)2 IgG (H+L) APC-conjugated secondary antibody. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin.