MA5-16168

antibody from Invitrogen Antibodies

Targeting: TP73 P73

Immunoprecipitation

Chromatin Immunoprecipitation

Antibody data

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Validation data

Product number: MA5-16168 - Provider product page
Provider: Invitrogen Antibodies
Product name: p73 Monoclonal Antibody (5B1288)
Antibody type: Monoclonal
Antigen: Other
Description: Suggested positive control: antigen standard for TP73 (transient overexpression lysate), p73 transfected cells..
Reactivity: Human, Mouse
Host: Mouse
Isotype: IgG
Antibody clone number: 5B1288
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: -20° C, Avoid Freeze/Thaw Cycles

TP73 protein structure - MA5-16168 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of p73 in HeLa cell lysate using a p73 (Alpha, Beta, Gamma, Delta Isoforms) monoclonal antibody (Product # MA5-16168) at1 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of p73 in HeLa cell lysate. Samples were incubated in p73 monoclonal antibody (Product # MA5-16168) using a dilution of 1 µg/mL.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of p73 in transfected cell lysate. Sample was incubated in p73 monoclonal antibody (Product # MA5-16168).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-p73 Pan Monoclonal Antibody (5B1288) (Product # MA5-16168) and a 59 kDa band corresponding to one of the isoform of Tumor protein p73 was observed in HCT 116 upon treatment with Etoposide. The expression of protein was also increased in HEK-293 upon Doxorubicin treatment. Nuclear enriched extracts (30 µg lysate) of HCT 116 (Lane 1) and HCT 116 treated with Etoposide (25 µM for 24hr) (Lane 2), HEK-293 (Lane 3) and HEK-293 treated with Doxorubicin (0.5 µM for 24hr) (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of p73 in Human breast cancer section. Samples were incubated in p73 monoclonal antibody (Product # MA5-16168) using a dilution of 10 µg/mL. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). The cancer cells showed cytoplasmic immunoreactivity for p73 and the signal was very weak in the core and stroma of tumors. Peripheral cells of tumor areas, apparently the myoepithelial cells, showed a strong nuclear staining of p73.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of p73 in Human breast cancer section. Samples were incubated in p73 monoclonal antibody (Product # MA5-16168) using a dilution of 10 µg/mL. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) with 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching using peroxide block. The sections were incubated with primary antibody for 30 minutes. Bond Polymer Refine Detection (Leica Biosystems) and DAB were used for signal detection which followed counterstaining with hematoxylin. Whole slide scanning and capturing of representative images (20X) were performed using Aperio AT2 (Leica Biosystems). The cancer cells showed cytoplasmic immunoreactivity for p73 and the signal was very weak in the core and stroma of tumors. Peripheral cells of tumor areas, apparently the myoepithelial cells, showed a strong nuclear staining of p73.