Explore
Validate
Learn
Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX630146 - Provider product page
- Provider
- GeneTex
- Product name
- Fumarate hydratase antibody [GT7612]
- Antibody type
- Monoclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
No comments: Submit comment
Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [GT7612] (GTX630146) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein by Western blot analysis.A. 50 ?g mouse liver lysate/extract10 % SDS-PAGEFumarate hydratase antibody [GT7612] (GTX630146) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein by Western blot analysis.A. 30 ?g 293T B. 30 ?g A431 C. 30 ?g HeLa D. 30 ?g HepG2 10 % SDS-PAGEFumarate hydratase antibody [GT7612] (GTX630146) dilution: 1:500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody [GT7612] (GTX630146) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 5 min.Green: Fumarate hydratase protein stained by Fumarate hydratase antibody [GT7612] (GTX630146) diluted at 1:500.Blue: Hoechst 33342 staining.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Fumarate hydratase antibody [GT7612] detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fumarate hydratase protein stained by Fumarate hydratase antibody [GT7612] (GTX630146) diluted at 1:200.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
