710020

antibody from Invitrogen Antibodies

Targeting: GRIN1 GluN1, NMDAR1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

710020

Provider

Invitrogen Antibodies

Product name

NMDAR1 Recombinant Polyclonal Antibody (1HCLC)

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

This antibody is predicted to react with mouse based on sequence homology.

Antibody clone number

1HCLC

Concentration

0.5 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NMDAR1 was performed by loading 20 µg of SH-SY5Y (lane1), Jurkat (lane2), U-87 MG (lane3), HeLa (lane4) and MCF7 (lane5) cell lysates using Novex®NuPAGE®4-12 % Bis-Tris gel (Product # NP0321BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Proteins were transferred to a PVDF membrane and blocked with 5 % skim milk for 1 hour at room temperature. NMDAR1 was detected at ~120 kDa using NMDAR1 Recombinant Rabbit Polyclonal Antibody (Product # 710020) at 1-3 µg/mL in 2.5 % skim milk at 4°C overnight on a rocking platform. Goat anti-Rabbit IgG-HRP Secondary Antibody (Product # G-21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western blotting Substrate (Product # 32106).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of NMDA Receptor 1 in rat brain lysate (30 µg/lane) (lane 1) and mouse brain lysate (30 µg/lane) (lane 2) using a NMDA Receptor 1 Recombinant Rabbit Polyclonal Antibody (Product # 710020) at a dilution of 5 µg/mL. NBT/BCIP was used as the substrate (Product # WB7105).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of NMDAR1 showing staining in the cytoplasm of paraffin-embedded human brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a NMDAR1 Recombinant Rabbit Polyclonal Antibody (Product # 710020) diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.