Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A010-31AP - Provider product page
- Provider
- Badrilla Ltd
- Product name
- RYR2 Phospho Serine-2814 (AP) IgG
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- The ryanodine receptor (RyR2) is a Ca2+ channel of cardiac muscle that plays a central role in EC coupling. The binding of Ca2+ to RyR2 opens the channel and Ca2+ stored in the SR moves through the channel into the cytosol to initiate muscle contraction. CaMKII was able to phosphorylate Ser-2814 of RYR2 enhancing Ca2+- sensitivity and increasing open probability. Excessive phosphorylation of Ser-2814 leads to atrial fibrillation, arrhythmias and sudden death.
- Host
- Rabbit
- Antigen sequence
TSQVS(PO3H2)VDAAH2819
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- Lyophilised: 4 °C Reconstituted:-80 °C
- Handling
- Reconstitute lyophilised powder in 100 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles
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Supportive validation
- Submitted by
- Badrilla Ltd (provider)
- Main image
- Experimental details
- Western blot
- Sample type
- Canine sarcoplasmic reticulum +/- CaMKII treatment
- Validation comment
- Extra non specific band at 140-150kDa
- Primary Ab dilution
- 0.736111111
- Secondary Ab dilution
- 1.430555556
- Protocol
- Protocol