12-1999-42

antibody from Invitrogen Antibodies

Targeting: CCR9 CDw199, GPR-9-6, GPR28

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

12-1999-42

Provider

Invitrogen Antibodies

Product name

CD199 (CCR9) Monoclonal Antibody (BBC3M4), PE, eBioscience™

Antibody type

Monoclonal

Antigen

Other

Description

Description: This BBC3M4 monoclonal antibody reacts with human CD199 (CCR9), a 7 transmembrane spanning G-protein-coupled receptor. CCR9 expression is regulated during thymic development and CCR9 deficiency results in mild impairment in thymocyte development. In the periphery, CCR9 and alpha 4 beta 7 integrin are upregulated in response to dendritic cell-derived retinoic acid, conferring preferential trafficking to gut-associated tissues. CCR9 is the receptor for CCL25 (TECK).

Conjugate

Yellow dye

Antibody clone number

BBC3M4

Concentration

5 µL/Test

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

CD4+ T cells isolated from normal human peripheral blood cells were stimulated for 3 days with all-trans retinoic acid, Anti-Human CD3, and Anti-Human CD28 Functional Grade Purifieds (Product # 16-0037-81 and Product # 16-0289-81). The cells were cultured for 2 additional days with all-trans retinoic acid and Human IL-2 Recombinant Protein (Product # 14-8029-81). These cells were surface stained with Anti-Human/Mouse Integrin beta 7 FITC (Product # 11-5867-42) and Mouse IgG1 K Isotype Control PE (Product # 12-4714-81) (left) or Anti-Human CD199 (CCR9) PE (right). Total viable cells were used for analysis.

Conjugate

Yellow dye