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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- R34757 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MIF Antibody
- Antibody type
- Polyclonal
- Antigen
- Amino acids NAANVGWNNSTFA were used as the immunogen for this MIF antibody.
- Description
- Antigen affinity purified antibody
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- Aliquot and store the MIF antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human thymus lysate with MIF antibody at 0.01ug/ml. Predicted molecular weight ~13kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot of Daudi (A) and Jurkat (B) lysate with MIF antibody at 0.01ug/ml. Predicted molecular weight ~13kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Sandwich ELISA using the MIF antibody at 1.5ug/ml as the detect.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE human thymus tissue with MIF antibody at 5ug/ml. Required HIER: steamed antigen retrieval with pH6 citrate buffer; AP-staining.
