NBP2-43674

antibody from Novus Biologicals

Targeting: CD3E

Immunocytochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP2-43674

Provider

Novus Biologicals

Product name

Mouse Monoclonal CD3 Antibody

Antibody type

Monoclonal

Description

Protein G purified.

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Vial size

0.1 ml

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD3 Antibody (13) [NBP2-43674] - Analysis of B6 / spleen Blue: Isotype control (anti-mouse IgG-FITC) dilution: 1:300 Red: CD3 antibody [0013] dilution: 1:50.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD3 Antibody (13) [NBP2-43674] - Analysis of Jurkat cell Black: Isotype control (ab18449) dilution: 1:50. Green: CD3 antibody [0013] dilution: 1:50.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD3 Antibody (13) [NBP2-43674] - An intracellular stain was performed on Jurkat cells with CD3 Antibody (13) NBP2-43674F (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to FITC.