Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-54405AF647 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal CD3 epsilon Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Rat, Bovine, Chicken/Avian, Porcine, Rabbit
- Host
- Mouse
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: CD3 epsilon Antibody (PC3/188A) [Alexa Fluor® 647] [NBP2-54405AF647] - An intracellular stain was performed on Jurkat cells with CD3 epsilon Antibody (PC3/188A) NBP2-54405AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.