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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- NB200-578 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB200-578, RRID:AB_10003082
- Product name
- Rat Monoclonal CD8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. NB200-578 recognises a non polymorphic epitope on the mouse CD8 alpha chain. Blocks MHC I dependent T cell responses in vitro and in vivo, and induces transplantation tolerance in combination with CD4 antibodies.
- Reactivity
- Human, Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 0.125 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references STAT3 labels a subpopulation of reactive astrocytes required for brain metastasis.
A novel combination immunotherapy for cancer by IL-13Rα2-targeted DNA vaccine and immunotoxin in murine tumor models.
Hypersensitivity and oral tolerance in the absence of a secretory immune system.
Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation.
Development of anti-PAX3 immune responses; a target for cancer immunotherapy.
Involvement of intestinal epithelial cells in dendritic cell recruitment during C. parvum infection.
Rats and mice exhibit distinct inflammatory reactions after spinal cord injury.
Linked suppression of skin graft rejection can operate through indirect recognition.
The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies.
Induction of tolerance in peripheral T cells with monoclonal antibodies.
Priego N, Zhu L, Monteiro C, Mulders M, Wasilewski D, Bindeman W, Doglio L, Martínez L, Martínez-Saez E, Ramón Y Cajal S, Megías D, Hernández-Encinas E, Blanco-Aparicio C, Martínez L, Zarzuela E, Muñoz J, Fustero-Torre C, Piñeiro-Yáñez E, Hernández-Laín A, Bertero L, Poli V, Sanchez-Martinez M, Menendez JA, Soffietti R, Bosch-Barrera J, Valiente M
Nature medicine 2018 Jul;24(7):1024-1035
Nature medicine 2018 Jul;24(7):1024-1035
A novel combination immunotherapy for cancer by IL-13Rα2-targeted DNA vaccine and immunotoxin in murine tumor models.
Nakashima H, Terabe M, Berzofsky JA, Husain SR, Puri RK
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):4935-46
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 15;187(10):4935-46
Hypersensitivity and oral tolerance in the absence of a secretory immune system.
Karlsson MR, Johansen FE, Kahu H, Macpherson A, Brandtzaeg P
Allergy 2010 May;65(5):561-70
Allergy 2010 May;65(5):561-70
Neonate intestinal immune response to CpG oligodeoxynucleotide stimulation.
Lacroix-Lamandé S, Rochereau N, Mancassola R, Barrier M, Clauzon A, Laurent F
PloS one 2009 Dec 14;4(12):e8291
PloS one 2009 Dec 14;4(12):e8291
Development of anti-PAX3 immune responses; a target for cancer immunotherapy.
Himoudi N, Nabarro S, Yan M, Gilmour K, Thrasher AJ, Anderson J
Cancer immunology, immunotherapy : CII 2007 Sep;56(9):1381-95
Cancer immunology, immunotherapy : CII 2007 Sep;56(9):1381-95
Involvement of intestinal epithelial cells in dendritic cell recruitment during C. parvum infection.
Auray G, Lacroix-Lamandé S, Mancassola R, Dimier-Poisson I, Laurent F
Microbes and infection 2007 Apr;9(5):574-82
Microbes and infection 2007 Apr;9(5):574-82
Rats and mice exhibit distinct inflammatory reactions after spinal cord injury.
Sroga JM, Jones TB, Kigerl KA, McGaughy VM, Popovich PG
The Journal of comparative neurology 2003 Jul 21;462(2):223-40
The Journal of comparative neurology 2003 Jul 21;462(2):223-40
Linked suppression of skin graft rejection can operate through indirect recognition.
Wise MP, Bemelman F, Cobbold SP, Waldmann H
Journal of immunology (Baltimore, Md. : 1950) 1998 Dec 1;161(11):5813-6
Journal of immunology (Baltimore, Md. : 1950) 1998 Dec 1;161(11):5813-6
The induction of skin graft tolerance in major histocompatibility complex-mismatched or primed recipients: primed T cells can be tolerized in the periphery with anti-CD4 and anti-CD8 antibodies.
Cobbold SP, Martin G, Waldmann H
European journal of immunology 1990 Dec;20(12):2747-55
European journal of immunology 1990 Dec;20(12):2747-55
Induction of tolerance in peripheral T cells with monoclonal antibodies.
Qin SX, Wise M, Cobbold SP, Leong L, Kong YC, Parnes JR, Waldmann H
European journal of immunology 1990 Dec;20(12):2737-45
European journal of immunology 1990 Dec;20(12):2737-45
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CD8 Antibody (YTS105.18) [NB200-578] - CD8 alpha Antibody (YTS105.18) [NB200-578] - Staining of a mouse lymph node cryosection with Rat anti mouse CD19, clone 6D5 (MCA1439), green in A and Rat anti Mouse CD8, red in B. Merged image in C wih nuclei counterstained blue using DAPI. Low power
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CD8 Antibody (YTS105.18) [NB200-578] - CD8 alpha Antibody (YTS105.18) [NB200-578] - Staining of Mouse lymph node cryosection.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Frozen: CD8 Antibody (YTS105.18) [NB200-578] - Published customer Image. Staining of (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: CD8 Antibody (YTS105.18) [NB200-578] - Staining of mouse spleen cells.
