Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- NB100-64922AF647 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal CD8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. Recognizes the human CD8 cell surface antigen, a 30/32kD glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells.
- Reactivity
- Human, Simian
- Host
- Rat
- Conjugate
- Red dye
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C in the dark.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Flow Cytometry: CD8 alpha Antibody (YTC182.20) [Alexa Fluor 647] [NB100-64922AF647] - A surface stain was performed on human peripheral blood lymphocytes with CD8 alpha (YTC128.20) antibody NB100-64922AF647 and a matched isotype control NBP2-21941AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-24867AF488.