NB100-64922

antibody from Novus Biologicals

Targeting: CD8A CD8

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

NB100-64922

Provider

Novus Biologicals

Proper citation

Novus Cat#NB100-64922, RRID:AB_962667

Product name

Rat Monoclonal CD8 Antibody

Antibody type

Monoclonal

Description

Protein G purified. Recognizes the human CD8 cell surface antigen, a 30/32kD glycoprotein expressed by the cytotoxic/suppressor subset of T lymphocytes, and more weakly by NK cells.

Reactivity

Human, Simian

Host

Rat

Isotype

IgG

Vial size

0.1 mg

Concentration

1.0 mg/ml

Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Flow cytometry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD8 alpha Antibody (YTC182.20) [NB100-64922] - Staining of human peripheral blood lymphocytes.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD8 alpha Antibody (YTC182.20) [NB100-64922] - Staining of human peripheral blood lymphocytes using CD8 alpha FITC.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Flow Cytometry: CD8 alpha Antibody (YTC182.20) [NB100-64922] - Using the Alexa Fluor 647 direct conjugate A surface stain was performed on human peripheral blood lymphocytes with CD8 alpha (YTC128.20) antibody NB100-64922AF647 and a matched isotype control NBP2-21941AF647. Cells were incubated in an antibody dilution of 2 ug/mL for 20 minutes at room temperature. A co-stain was performed with NBP2-24867AF488.