MA1-10135

antibody from Invitrogen Antibodies

Targeting: MS4A1 B1, Bp35, CD20, MS4A2

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-10135

Provider

Invitrogen Antibodies

Product name

CD20 Monoclonal Antibody (2H7)

Antibody type

Monoclonal

Antigen

Other

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

2H7

Vial size

100 µg

Concentration

1.0 mg/mL

Storage

4° C, do not freeze

References

Submitted references

Longitudinal analyses reveal distinct immune response landscapes in lung and intestinal tissues from SARS-CoV-2-infected rhesus macaques.

Zheng H, Chen Y, Li J, Li H, Zhao X, Li J, Yang F, Li Y, Liu C, Qin L, Zuo Y, Zhang Q, He Z, Shi H, Li Q, Liu L
Cell reports 2022 May 24;39(8):110864

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Mucosal Inducible NO Synthase-Producing IgA+ Plasma Cells in Helicobacter pylori-Infected Patients.

Neumann L, Mueller M, Moos V, Heller F, Meyer TF, Loddenkemper C, Bojarski C, Fehlings M, Doerner T, Allers K, Aebischer T, Ignatius R, Schneider T
Journal of immunology (Baltimore, Md. : 1950) 2016 Sep 1;197(5):1801-8

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Effects of B Cell Depletion on Early Mycobacterium tuberculosis Infection in Cynomolgus Macaques.

Phuah J, Wong EA, Gideon HP, Maiello P, Coleman MT, Hendricks MR, Ruden R, Cirrincione LR, Chan J, Lin PL, Flynn JL
Infection and immunity 2016 May;84(5):1301-1311

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Evidence for innate immune system activation in HIV type 1-infected elite controllers.

Krishnan S, Wilson EM, Sheikh V, Rupert A, Mendoza D, Yang J, Lempicki R, Migueles SA, Sereti I
The Journal of infectious diseases 2014 Mar;209(6):931-9

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CD20 was performed using 70% confluent log phase Ramos cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CD20 Monoclonal Antibody (2H7) (Product # MA1-10135) at 5 µg/mL concentration in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32766), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing plasma membrane and cytoplasm localization. Panel e represents negative cell line Jurkat. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry surface staining of human peripheral blood with anti-CD20 monoclonal antibody (Product # MA1-10135).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD20 (2H7) purified Monoclonal antibody (Product # MA1-10135); concentration in sample 0.6 µg/mL, GAM APC.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Separation of human CD20 positive lymphocytes (red-filled) from neutrofil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD20 (2H7) purified Monoclonal antibody (Product # MA1-10135); concentration in sample 0.6 µg/mL, GAM APC.